摘要
目的研究北京市2006年新确认HIV-1感染者毒株的耐药突变本底数据。方法随机选取北京市2006年新确认HIV-1感染者抗凝全血标本50份，提取血浆病毒RNA，用逆转录聚合酶链反应扩增HIV-1 pol区基因片段，并进行序列测定及耐药基因型分析。结果成功扩增出34份标本的pol区基因；在1例样本的蛋白酶编码区检测出1个主要耐药突变，7例样本检测出7个次要耐药突变，主要耐药突变为M46L，毒株是CRF01_AE亚型，次要耐药突变有4种，出现的频率分别为A71T（2个）、A71V（3个）、Q58E（1个）、V11IV（1个）。在14例样本逆转录酶编码区检测出一种或多种核苷类和（或）非核苷类逆转录酶抑制剂耐药突变，9例标本检出核苷类逆转录酶抑制剂耐药突变，出现频率分别为：V118I（42．9％）、M184V（7．1％）、A62V（7．1％）、K70T（7．1％）、K65R（7．1％）、K219N（7．1％）、T69d（7．1％）、V75LV（7．1％）、K219R（7．1％）；10例标本检出核苷类逆转录酶抑制剂耐药突变，出现的频率分别为V106I（35．5％）、Y181C（15．4％）、K103KR（7．7％）、K103R（7．7％）、L100LV（7．7％）、V108I（7．7％）、V179D（7．7％）、V179DV（7．7％）。结论北京市2006年新确认HIV-1感染者毒株中已经存在一定比例耐药突变，有必要定期进行耐药性监测研究。
Objective To study the drug resistance mutations of reverse transcriptase and protease among HIV-1 strains of new confirmed HIV-1-infected patients in Beijing, provide the base-line data for clinical antiviral treatment in Beijing. Methods We amplified partial pal sequences by RT-PCR and nest-PCR, then sequenced the gene segment and phylogenetically analyzed. Drug-resistance associated mutations in protease and reverse transcriptase regions were analyzed with Stanford University HIV Drug Resistance Database. Results Total of 34 pol gene sequences were obtained from 50 HIV-1-infected patients. Analysis of drug-resistance associated mutation showed that 15 of 34 persons infected with drug-resistant HIV-1, among which major mutations in PR and RT regions were 1 and 14 of 34 persons respectively. The frequencies of major mutation in PR region were M46L （ 100% ） and the NRTI （ nueleoside reverse ranseriptase inhibitor） and NNRTI（non-nucleoside reverse ranscriptase inhibitor） resistance mutations in RT region were V118I （42.9%）, M184V（7.1%）, A62V（7. 1%）, K70T（7. 1%）, K65R（7. 1%）, K219N（7. 1%）, T69d （7.1%）, V75LV（7.1% ）, K219R（7.1%） and V106I（35.5% ）, Y181C （15.4%）, K103KR（7.7% ）, K103R（7.7% ）, L100LV（7.7% ）, V1081（7.7% ）, V179D（7.7% ）, V179DV（7.7% ）, respectively. Conclusion There were drug resistance mutations in some new confirmed HIV-l-infectcd patient in this study, the surveillance of HIV-1 drug resistance mutations should be paid more attention.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2009年第10期886-889,共4页
Chinese Journal of Microbiology and Immunology
