摘要
为了探讨化学杂交剂对光温敏核不育水稻雄性不育的影响效果，１９９３～１９９６年对光温敏核不育水稻衡农Ｓ－１和培矮６４Ｓ进行了化学杂交剂筛选和应用技术的研究．结果表明，湖南农业大学生化教研室研制的无毒化学杂交剂“保纯灵”在光温敏核不育水稻衡农Ｓ－１和培矮６４Ｓ可育状态下叶面喷施（适宜时期为花粉充实早期），诱导雄性不育效果较好，辅助制种能明显提高杂种纯度，在衡农Ｓ－１和培矮６４Ｓ育性恢复、自交结实率２０％左右时，每６６６．７ｍ２分别用“保纯灵”１６０和２４０ｇ，对水２００ｋｇ叶面喷施１次，一般空壳率达９８％，杂种纯度９５％左右；Ｆ１杂交种子发芽率正常，秧苗生长旺盛；根芽比提高，细胞色素氧化酶、过氧化物酶和多酚氧化酶活性增强；“保纯灵”对水稻杀雄有效的剂量范围广，活性期长，处理后水稻开花集中，午前花比例提高，雌蕊柱头生活力正常．但处理后的水稻植株对赤霉素有轻度钝感，生育期延长２～３ｄ．
An experiment was conducted on thermo photo sensitive genic male sterility rice (TPGMSR),Hengnong S 1 and Pei′ai 64S.This experiment,from 1993 to 1996,is aimed at the study of the TPGMSR′S selection of chemical hybridizing agent and its amplication technology. The main results read as follows. Optimum time for leaf spray of Baochunling,a non poisonous chemical hybridizing agent produced by the Biochemical Teaching and Research office of HNAU,is at the early stage of pollen filling.Baochunling (BCL) has a significant inducing effect on TPGMSR′S male sterility. As an auxiliary to the production of hybrid seeds,a noticeable raise can been seen in the purity of a hybrid.When Hengnong S 1 and Pei′ai 64S′ fertility restores,and they have 20% of self crossed seed setting,the optimum amount of BCL applied by leaf spray is 2.4 kg per hectare mixed with 200 kgof water and 3.6 kg per hectare mixed with 200 kgof water respectively. After 6 sprays on leaves,they commonly produce 98% of male sterility and its purity of hybrid seed reaches 95%. Germination percentage of hybrid seed F 1 is normal and its seedlings grow promisingly. Correspondingly,the rate of radical buds has been raised,the activity of COX,POD and polyphenol oxidase are strengthened. The dose of BCL varies in a wide range. It keeps a rather long activity period. After the treatment,the rice blooms earlier and intensively. It appears a normal stigma receptivity. BCL slightly reduces rice′s sensitivity to GA and its seed bearing period is delayed 2￣3 days.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
1998年第4期270-276,共7页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科委攻关课题
关键词
水稻
水温敏核不育
化学杂交剂
制种
应用技术
paddy thermo photo sensitive genic male sterility chemical hybridizing agent production of hybrid seed
