摘要
为探讨外源性二氧化硫(sulfur dioxide,SO2)对脂多糖(lipopolysaccharide,LPS)所致大鼠肺损伤时肺组织炎性反应的影响,采用经尾静脉注射LPS的方法复制大鼠肺损伤模型,将雄性Sprague-Dawley(SD)大鼠随机分为4组:对照组、LPS组、LPS+SO2组[注射LPS前10min静脉给予外源性SO2供体亚硫酸钠/亚硫酸氢钠(Na2SO3/NaHSO3)0.5mL]和SO2组(单纯给予Na2SO3/NaHSO3)。给药后6h处死大鼠,测定肺系数;光镜下观察肺组织形态学改变;测定支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中的中性粒细胞(polymorphonuclear neutrophil,PMN)数量;采用流式细胞术检测肺组织中细胞间黏附因子-1(ICAM-1)的表达情况;应用酶联免疫法(ELISA)检测血清中IL-1、IL-6和IL-10含量。所得结果如下:与对照组比较,静脉注射LPS可引起肺组织明显的形态学改变;肺系数、肺损伤的组织学定量评价指标(IQA)和BALF中PMN数量增加;肺组织中ICAM-1表达增多,血清中IL-1、IL-6和IL-10水平升高。预先给予Na2SO3/NaHSO3可显著减轻LPS所致肺损伤的程度,同时使肺系数、肺IQA和BALF中PMN数量减少;肺组织中ICAM-1表达下降,血清中IL-1、IL-6水平下降而IL-10水平升高。SO2组大鼠上述各项指标与对照组大鼠相比无显著差异。以上结果提示,外源性SO2可抑制LPS所致大鼠肺损伤时肺组织中的炎症反应。
To investigate the influence of sulfur dioxide （SO2） on lipopolysaccharide （LPS）-induced acute lung injury （ALI）, we examined the influence of exogenous SO2 on pulmonary tissue inflammatory response. A rat model of ALl induced by intravenous （IV） injection of LPS was developed. Male Sprague-Dawley （SD） rats were divided into four groups randomly： control group, LPS group, LPS plus SO2 group （IV injection of 0.5 mL Na2SO3/NaHSO3 10 min before LPS administration） and SO2 group （only given NaESO3/NaHSO3）. Animals were sacrificed 6 h after agent administration. Lung weight/body weight ratio （LW/BW） was measured and calculated. Morphological changes of lung tissues were observed. The number of polymorphonuclear neutrophil （PMN） in the bronchoalveolar lavage fluid （BALF）, intercellular adhesion factor-1 （ICAM-1） expression in the lung tissue and IL-1, IL-6 and IL-10 levels in the serum were tested. The results showed that, compared to control rats, the LPS-treated rats had severe injuries of lung tissues and an increased LW/BW, increased index of quantitative assessment （IQA） score, increased PMN number in the BALF, increased ICAM-1 expression in the lung tissue and increased IL-1, IL-6 and IL-10 levels in the serum 6 h after LPS injection. Administration of the SO2 donor, Na2SO3/NaHSO3, into LPS-treated rats reduced the LW/BW, PMN number and ICAM-1 expression, and alleviated the degree of ALI （measured by the IQA score）. In addition, Na2SO3/NaHSO3 decreased IL-1 and IL-6 levels, but increased IL-10 level in the serum. There were no significant differences in the above indexes between SO2-treated rats and control rats. These results suggest that exogenous SO2 could inhibit the pulmonary tissue inflammatory response in rats with LPS-induced ALI.
出处
《生理学报》
CAS
CSCD
北大核心
2009年第5期499-503,共5页
Acta Physiologica Sinica
基金
supported by the National Natural Science Foundation of China(No.30800440)
the Natural Science Foundation of Hebei Province(No.C2007000830
C2008001040)
the Natural Science Foundation of Beijing(No.7092035)
the Educational Committee of Hebei Province(No.2005123)
关键词
内毒素
肺损伤
二氧化硫
大鼠
endotoxin
lung injury
sulfur dioxide
rat
