摘要
目的探讨白细胞介素10(interleukin-10,IL-10)启动子区域基因多态性与广泛型侵袭性牙周炎易感性的关系。方法收集30例广泛型侵袭性牙周炎患者和30名健康对照者的颊黏膜拭子,提取基因组DNA,采用PCR-RFLP方法检测IL-10基因启动子-1082G/A、-819C/T、-592A/C位点基因多态性,比较广泛型侵袭性牙周炎患者和健康对照组中等位基因频率和基因型分布。结果IL-10-1082G/A、-819C/T、-592A/C位点等位基因频率和基因型分布在患者和对照组之间差异无统计学意义(P>0.05)。结论IL-10启动子区基因多态性与汉族人群广泛型侵袭性牙周炎易感性无明显相关关系。
Objective To evaluate the polymorphisms at positions -1082G/A, -819C/T and -592A/C within the IL-10 gene promoter in patients with generalized aggressive periodontitis （n = 30） in comparison with periodontitis-free controis （n = 30） in Han nationality in Guangdong area. Methods Genomic DNA was obtained from 30 GAP. patients and 30 controls. Interleukin-10 promoter polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphisms （PCR-RFLP）. Distributions of single alleles and genotypes were calculated by the chi-square test. Results We found no significant difference in allele or haplotype frequencies between patients and controls. Conclusion The results suggest that IL-10 gene polymorphisms are not associated with the susceptibility to generalized aggressive periodontitis in Han nationality in Guangdong area.
出处
《广东牙病防治》
2009年第10期472-475,共4页
Journal of Dental Prevention and Treatment
基金
广东省医学科学技术研究基金(B2008014)
关键词
牙周炎易感性
IL-10基因多态性
侵袭性牙周炎
汉族
Susceptibitity to periodontitis
IL-10 gene polymoephisms
Aggressive periodontitis
Han nationality
