摘要
采用日本血吸虫感染动物制备小白鼠病理性肝纤维化模型，并观察水蛭桃仁汤对早期血吸虫性肝纤维化病理过程的阻断及逆转作用。中药治疗组动物血清Ⅰ型前胶原（ＰＣ－Ⅰ）及血清Ⅲ型前胶原（ＰＣ－Ⅲ）含量减少，血清Ⅲ型前胶原与对照组比较有显著性差异（Ｐ＜０．０５）；动物血清中肿瘤坏死因子（ＴＮＦ－α）及白细胞介素６（ＴＬ－６）均低于实验对照组（Ｐ＜０．０５）；肝组织中羟脯氨酸（ＨＹｐ）含量下降（Ｐ＜０．０５）。图象分析结果表明，水蛭桃仁汤能使日本血吸虫性肝虫卵肉芽肿的周长、最大直径及最小直径缩小，肝内病灶中网状纤维减少，增生纤维组织所占面积下降，肝病灶炎性细胞浸润明显地减轻。提示水蛭桃仁汤对减轻日本血吸虫病所致的肝纤维化损伤有良好的临床前景。
Morphometric techniques,RIA and histochemical techniques were applied to study the anti hepatic fibrosis by Hirudin and Prunus Persica.The results showed that:the percentage of the area of reticular fiber in egg granuloma was smaller than that of contol group ( P <0.01).The perimeter,long and short diameter of the egg granuloma in the experimental mice was decreased ( P <0.05).The results indicated that PC Ⅲ and PC Ⅰ in the experimental group was decreased in comparison with control group and HYp was decreased in the treated group( P <0.05).The above mentioned results provided an important basis for treatment of hepatic fibrosis induced by various factors,especially by parasite infection.
基金
冶金工业部自然科学研究基金
