摘要
目的:应用荧光原位杂交技术检测乳腺癌组织人表皮生长因子受体2(human epidermal growth factor receptor 2,Her-2)基因扩增情况。并对操作方法进行优化。方法:收集2008年4月至2008年10月期间我院乳甲科进行手术治疗的乳腺癌患者的癌组织病理切片,采用荧光原位杂交(FISH)技术检测Her-2基因扩增情况,并与免疫组化结果相比较,分析两者的相关性,同时优化操作方法。结果:收集的40例乳腺癌石蜡切片中,免疫组化检测Her-2蛋白表达(+++)有6例,(++)有22例,(+)有7例,(-)有5例。其中Her-2蛋白表达(+++)的标本FISH检测结果均为阳性,基因扩增与蛋白表达的符合率为100%;Her-2蛋白表达(++)的标本FISH结果有6例阳性,16例阴性,基因扩增与蛋白表达的符合率为27.3%。Her-2蛋白表达(+)及(-)的标本FISH结果均为阴性。Her-2蛋白表达(++)及以上的标本基因扩增与蛋白表达的符合率达到42.9%(r=0.584,P<0.01)。结论:FISH检测乳腺癌Her-2基因扩增的结果与IHC检测的蛋白表达结果符合率较好,可作为乳腺癌Her-2基因检测的一项新技术;在具体操作时,应注意严格控制切片厚度、消化时间及变性杂交温度等因素。
Objective To apply dual-color fluorescence in situ hybridization for detection of the expressions of Her- 2 gene in breast carcinoma tissues, and to find some modifications of the FISH method. Methods To collect the pathological section of breast cancer tissues from 40 patients with primary infiltrating ductal carcinomas who were received surgery in our hospital from April 2008 to October 2008. Her-2 gene amplification were detected by FISH and compared with the results of IHC. The correlation between these results was analyzed and optimization to FISH was discussed. Results In the 40 tissue specimens of breast carcinoma, IHC detected 6 specimens with Her-2 score （＋＋＋）, 22 specimens with score （＋＋）, 7 specimens with score （＋） and 5 specimens score negative. FISH identified gene amplification in 100% （6/6） specimens with an IHC score （＋＋＋）, and in 27.3% （6/22）specimens with an IHC score （＋＋）, thus the concordant ratio between IHC and FISH was 42.9%（12/28）. The two results showed significant relation（r = 0.584,P 〈 0.01 ） o The negative result were in all specimens with an IHC score （＋） and （-）. Conclusion The results of Her-2 gene amplification detected by FISH have well concordance with the protein expression results detected by IHC .As a new method for detection of Her-2 gene, the thickness of the sections , the post-hybridization washing temperature and time should be strictly controlled.
出处
《实用医学杂志》
CAS
北大核心
2009年第21期3685-3687,共3页
The Journal of Practical Medicine
