摘要
为估价雌激素、雌激素受体(ER)对慢性鼻窦炎发生的作用,确定ER在鼻窦粘膜的分布部位,本文用鼠抗人单克隆抗ER抗体,对61例慢性鼻窦炎患者的前组筛窦粘膜,2例正常筛窦粘膜和10例正常鼻粘膜的石蜡标本行SLAB法免疫组化染色,同时作常规HE染色、甲苯胺蓝染色。结果发现ER主要在部分粘膜下腺体的腺泡和腺管上皮细胞表达,慢性鼻窦炎组筛窦粘膜ER阳性率为55.7％,2例正常鼻窦粘膜内未见ER阳性细胞,10例正常鼻腔粘膜ER阳性率为20％,ER阳性组嗜酸性细胞和肥大细胞浸润程度明显高于ER阴性组。结果提示雌激素、雌激素受体对慢性鼻窦炎发病有一定作用。
Estrogen has been demonstrated to play a role in chronic nasal inflammation. Its action at the cellular level begins with binding to the highly specific protein receptor molecules called estrogen receptors (ER). To evaluate the effect of estrogen on the pathogenesis of chronic sinusitis and localize the specific receptor-containing cell type, the expression of ER in sinusal mucosa on patients with sinusitis (61), normal nasal mucosa (10), and healthy sinusal mucosa (2) was investigated hy using immunocytochemical (SLAB) method. Monoclonal antibodies specific to ER were used. HE and toluidine blue staining were also used to detect eosinophilia and mast cell. The results showed that the ER positive rates were 55.7% in chronic sinusitis, 20% in normal nasal mucosa and no ER-positive in healthy sinusal mucosa. Positive reactivity was limited to the acini and glandular duct, eosinophilia and mast cell under the epithelium of sinusal mucosa of chronic sinusitis in ER-positive cases were more than those in ER-negative (P<0.05). It is suggests that estrogen and ER play certain role in the development of chronic sinusitis. [-Chinese Arch Otolaryngol Head Neck Surg, 1998; 5(4): 239～243 From the Beijing Institute of Otorhinolaryngology, Beijing 100005 (Dr. Liu Xiangyan]
出处
《耳鼻咽喉（头颈外科）》
1998年第4期239-243,共5页
Chinese Arch Otolaryngology-Head Neck Surg
