摘要
目的探讨炎症反应在高血压左室重构中的作用及中药降压脉净液对其干预作用与机制。方法选用自发性高血压大鼠(SHR),8周龄40只,随机分为4组,A组:正常对照组(SD)、B组:模型组(SHR)、C组:降压脉净液+络活喜(治疗组)、D组:络活喜(西药对照组)、E组:牛黄降压丸+络活喜(中药对照组)。灌胃治疗,疗程20周。观察各组血压及测定心脏重量指数(HWI);观察病理学改变,测算心肌间质胶原容积分数(CVF)、血管周围胶原面积/血管腔横断面积(PVCA)及采用放射免疫法检测大鼠血清中白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)的表达水平。结果①降压脉净液+络活喜可显著降低SHR心肌细胞肥大、心肌间质炎性细胞浸润及胶原含量P<0.01,也有降低心脏重量指数的趋势。②SHR血清中TNF-α、IL-6水平表达较正常大鼠显著升高,P<0.01;③降压脉净液+络活喜与模型对照组比较可明显降低SHR血清中IL-6水平,P<0.01,优于其他给药组。结论SHR血清中IL-6、TNF-α的过度表达可能参与了高血压左室重构的过程。中药降压脉净液具有独立于降压之外的逆转SHR左室重构的作用,其机制可能与降低了IL-6的表达水平有关。
Objective To investigate the influence of inflammatory reaction on the left ventricular remodeling induced by spontaneously hypertension, and to discuss the intervene of Jiangyamaijing Solution. Methods The rats with spontaneously hypertension （8 weeks old, n = 40） were randomly divided into the normal control group （group A）, model group （group B）, treatment group treated with Jiangyamaijing Solution and Luohuoxi Tablets orally （group C）, Western medicine control group treated with Luohuoxi Tablets orally （group D） and Chinese medicine control group treated with Niuhuangjiangya Pills and Luohuoxi Tablets orally （group E）. One treatment course was 20 weeks. The blood pressure and pathological changes were observed, the heart weight index （HWI）, myocardial interstitial collagen volumetric fraction （CVF） and perivascular collagen area （PVCA） were calculated, and the expressions of serum interleuking-6 （IL-6） and tumor necrosis factor-α （TNF-α） were detected by using radioimmunoassay. Results inflammatory cell infiltration HWI had a trend of descent. ①In group D the cardiomyocyte hypertrophy and myocardial interstitial were relieved and collagen content was reduced significantly （ P 〈 0.01 ）. ②The expressions of serum IL-6 and TNF-α were higher significantly （ P 〈0.01 ） in rats with spontaneously hypertension than those in normal rats. ③In group D the expression of serum IL-6 decreased significantly compared with that in group B and the effect was better than that in other groups （ P 〈 0.01 ）. Conclusions The excess expressions of serum IL-6 and TNF-α may be involved in the left ventricular remodeling induced by spontaneously hypertension. The remodeling can be reversed by Jiangyamaijing Solution and the mechanism may be related to the decrease of IL-6 expression.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2009年第10期695-698,705,I0002,共6页
Journal of Beijing University of Traditional Chinese Medicine
基金
中日友好医院院级课题资助项目(No.20053077)
关键词
高血压病
降压脉净液
左室重构
白细胞介素-6
肿瘤坏死因子Α
大鼠
hypertension
Jiangyamaijing Solution
left ventricular remodeling
interleuking-6
tumor necrosis factor-α
rats
