摘要
目的探讨卵泡液中CD56+自然杀伤(NK)细胞及活化的CD56+NK(CD56+CD69+NK)细胞的比例与体外受精(IVF)结局的关系。方法应用流式细胞仪分析成熟卵泡液中CD56+NK细胞及活化的CD56+NK细胞的比例,结合IVF治疗结果,探讨其相关性。结果妊娠患者的卵泡液中平均CD56+NK细胞及活化的CD56+NK细胞分别为(15.57±3.10)%和(2.63±0.94)%,未妊娠患者的卵泡液中平均CD56+NK细胞及活化的CD56+NK细胞分别为(19.12±5.37)%和(4.06±2.08)%,显著高于妊娠组(P<0.05)。结论接受IVF助孕治疗患者中成熟卵泡液中CD56+NK细胞及活化的CD56+NK细胞的比例较低者易获临床妊娠。
Objective To investigate the proportion of follicular fluid CD56 ＋ natural killer （NK） cells to the total lymphocytes and the ac- tivated CD56＋ NK cells to the total CD56＋ NK cells. To provide an evidence for improving the chnical pregnancy rate in in vitro fertilization （WF） treatment by regulating the function of NK cells. Melhods Triple color flow cytometry was used to analyze the proportion of CD56＋ NK cells and activated CD56＋ NK cells in mature follicular fluid, The IVF treatment outcome was closely followed up. The relationship between the proportion of CD56＋ NK cells and activated CD56＋ NK cells and the IVF treatment outcome was analyzed statistically. Results The proportion of CD56＋ NK cells and activated CD56＋ NK cells in mature follicular fluid of women who got pregnancy by IVF treatment was （15.57±3.10）% and （2.63±0.94）% respectively,while the proportion of the women who didn＇t get pregnancy was （19.12±5.37）% and （4.06±2.08）% respectively, which was significantly higher than the pregnant group（ P 〈 0.05 ）. Conclusion The women with lower proportion of CD56＋ NK cells and activated CD56＋NK cells in mature follicular fluid of is easier to get pregnancy by/VF. An altered NK cells profile in follicular fluid could therefore influence fertility in IVF treatment outcome.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2009年第10期770-772,共3页
Journal of China Medical University
基金
辽宁省自然科学基金资助项目(2007118)
关键词
体外受精
自然杀伤细胞
卵泡液
流式细胞术
in vitro fertilization
natural killer cell
follicular fluid
triple color flow cytometry
