摘要
目的观察HA14-1是否有诱导体外小鼠Lewis肺癌(LLC)细胞凋亡作用。方法将LLC细胞培养传代,细胞分为空白组和实验组,空白组只加培养液,实验组分别加入5种不同浓度的HA14-1(12.5,25,50,75,100μmol/L),分别作用24,48,72h,MTT法测定LLC细胞存活分数,流式细胞学方法测定LLC细胞凋亡情况、免疫组织化学方法测定LLC细胞Bcl-2蛋白的表达情况及HA14-1作用前后Bcl-2蛋白的表达情况。结果两组细胞中,与空白组比较,不同浓度的HA14-1实验组作用LLC细胞后,细胞存活率随浓度增加和时间延长越来越低,细胞的凋亡率随着浓度的增加有增加趋势,其差异在统计学上有显著性意义(P<0.01);免疫组织化学结果显示LLC细胞高表达Bcl-2,且HA14-1作用后Bcl-2蛋白的表达减少。结论HA14-1有促小鼠Lewis肺癌细胞凋亡的作用。
Objective To observe the effect of HA14-1 on the apoptosis of Lewis lung cancer （LLC） cells in vitro. Methods Cultured LLC cells were divided into experimental group and control group. Different concentrations of HA14-1 （12.5,25,50,75,100 mmol/L） was added into the cells of experimental groups respectively for 24,48,72 hours. The LLC cell survival fraction, apoptotic rate and Bcl-2 expres- sion before and after HA14-1 treatment were detected by MTr,flowcytometry and immunohistochemistry respectively. Results Compared with the blank control group,the cell survival rate of the experimental group declined with the increase of HA14-1 concentration,while the apoptotic rate increased with the concentration of HA14-1 （P 〈 0.01 ）;the results of immunohistochemistry showed that Bcl-2 was expressed strongly in LLC cells and HA14-1 induced a decrease of Bcl-2 expression in LLC cells. Conclusion HA14-1 may promote the apoptosis of LIE cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2009年第9期668-670,共3页
Journal of China Medical University
基金
辽宁省教育厅科学研究计划项目(20060965)
