摘要
目的研究不同波长光照对草履虫增殖的影响,克隆草履虫感光蛋白基因。方法以双小核草履虫Par-amecium aureli为研究对象,分别置于黄色光(578～592nm)、蓝色光(446～464nm)、红色光(620～760nm)、白光和自然光下,每隔1h随机抽样法显微观察并计数;RT-PCR克隆草履虫感光蛋白基因。结果不同波长光的照射下,与自然光比较,第1天黄光组草履虫增殖显著上升(P<0.01),蓝光组和红光组草履虫增殖受到不同程度的抑制(P<0.01,P<0.05),白光组无明显差异(P>0.05);第2天,蓝光组、红光组和白光组草履虫增殖仍受到抑制(P<0.01),黄光组作用不显著(P>0.05)。黄光组和白光组草履虫总RNA作为模板,克隆出大小约500bp的rhodop-sin-like基因cDNA片段,5个不同光照组均克隆出大小约195bp的Longwave sensitive opsin-like基因cDNA片段。结论黄色光显著地促进草履虫增殖,蓝色光和红色光抑制其增殖;黄光和白光能诱导草履虫rhodopsin-like基因表达;Longwavesensitiveopsin-like基因在草履虫有表达。
Objective To investigate the effect of different wavelengths of light on the reproduction rate of Paramecium aureli. To clone genes encoding possible photosensitive receptor proteins. Methods Reproduction of Paramecium aureli was studied under different illumination conditions： yellow light （wave length 578 - 592 nm）, blue light （wave length 446 464 nm）, red light （wave length 620 -760 nm）, white light, and shaded sunlight. Samples were extracted randomly from each group every hour and the the quantity counted under a microscope. RT-PCR was employed to clone genes encoding possible photosensitive receptor proteins. Results Under light of different wavelengths, paramecium in the yellow light group propagated fastest and showed great discrepancy compared to other groups （ P 〈 0.01 ）, the reproduction rate in the blue and red groups were suppressed （P 〈 0. 01, P 〈 0.05 ）. During the second day, suppression in the blue and red groups continued （P 〈 0.01 ）, but yellow group showed little when discrepancy compared to other groups （ P 〉 0.05 ）. cDNA fragments of about 500 bp were cloned from RNA templates from the yellow and white groups, cDNA fragments of long wave sensitive opsin-like gene, about 195 bp in length, were cloned from 5 different groups. Conclusions Yellow light promoted reproduction of paramecium while red and blue light suppressed reproduction; yellow and white light may up-regulate the expression of gene encoding rhodopsin-like protein in Paramecium aureli; long wave sensitive opsin-like gene might be expressed in paramecium
出处
《四川动物》
CSCD
北大核心
2009年第6期821-826,共6页
Sichuan Journal of Zoology
