摘要
目的：观察创伤后应激障碍（PTSD）大鼠海马神经元凋亡和细胞色素C（CytC）的表达与释放，探讨细胞凋亡与Cyt C的关系。方法：采用单一延长应激（SPS）方法刺激大鼠建立PTSD大鼠模型，取SPS刺激后4、7、14、28d组和正常对照组。用原位末端标记法观察神经元凋亡，免疫组织化学和免疫印迹法检测CytC蛋白的表达，酶电镜组织化学术观察CytC的释放。结果：SPS刺激后4d线粒体肿胀，外膜破裂，CytC释放。胞质中CytC蛋白于SPS刺激后4d达到高峰并维持较高水平，SPS刺激后7d逐渐下降。凋亡细胞于SPS刺激后7d达高峰。结论：CytC从线粒体释放人胞质是引发PTSD大鼠海马神经元凋亡的关键步骤。
Objective： To observe changes of apoptosis and cytochrome C expression and release in hippocampus of rat model of posttraumatic stress disorder （PTSD）, and further explore their relationship. Methods： Single prolonged stress （SPS） was used to duplicate a rat model of PSTD. The rats undergoing SPS were analyzed at day 4, 7, 14 and 28 by using TUNEL for apoptosis of hippocampal neurons,immunohistoehemical staining and Western blot for expression of cytochrome C, and electron microscopic enzyme histochemistry for release of cytochrome C in mitochondria. Results： Four days after SPS, the cytochrome C was released from mitochondria to cytoplasm and its expression was up to peak until the 7th day, when the expression of cytochrome C decreased gradually. Apoptosis in hippoeampus was peaked at day 7. Conclusion： Cytochrome C may play an important regulatory role in cellular apoptosis in the rat hippoeampus of PTSD.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第5期651-653,659,共4页
Chinese Journal of Anatomy
基金
国家自然科学基金（30600341）
关键词
创伤后应激障碍
细胞色素C
凋亡
海马
大鼠
posttraumatic stress disorder
cytoehrome C
apoptosis
hippocampus
rat
