摘要
目的观察脐带血清体外培养人骨髓间充质干细胞的生物学特性。方法抽取骨髓穿刺液10ml，采用含10％脐带血清的DMEM／F12培养，流式细胞仪检测细胞表面抗原，成骨诱导体系及脂肪诱导体系诱导BM—MSCs定向分化。结果BM—MSC—Ss高表达CD29、CD73、CD105，不表达CD34、CIM5、CD31，BM—MSCs体外能诱导为成骨细胞和脂肪细胞。结论脐带血清培养的BM—MSCs的具有较高的纯度和体外分化能力，脐带血清培养BM—MSCs适合临床应用。
Objective To observe biological characteristics of adult human marrow mesenchymal stem cells （BM-MSCs） cuhured by the umbilical cord serum. Methods BM-MSCs were obtained from fresh adult human bone marrow and cultured with DMEM/F12 which included 10% umbilical cord blood serum. The surface antigens of BM-MSCs were detected by flow cytometry. BM-MSCs were cultured into differentiation of osteoblasts and adipocytes with conditioned medium for osteogenesis and adipogenesis. Results The antigen of CD29, CD73, and CD105 were high expressed on BM-MSCs. The antigen of CD34, CD45 and CD31 were not expressed on BM -MSCs. The BM- MSCs were differentiated into osteoblasts and adipocytes. Conclusion BM-MSCs have high purity and differentiation potency under culturing with umbilical cord blood serum. The umbilical cord serum is adapted to clinical application.
出处
《中国医师杂志》
CAS
2009年第10期1317-1320,共4页
Journal of Chinese Physician
基金
基金项目：湖南省科技厅重点支持项目（06SK2007）
关键词
胎血
细胞培养技术/方法
造血干细胞
骨髓细胞
Fetal blood
Cell culture techniques/MT
Hematopoietic stem cells
Bone marrow cells
