摘要
目的:建立以高效液相色谱法测定人血浆中比阿培南浓度的方法。方法:色谱柱为Hypersil ODS,流动相为乙腈-0.1mol.L-1乙酸钠缓冲液(2:98,pH4.38),流速为1.0mL.min-1,检测波长为300nm,柱温为25℃,进样量为20μL,样品温度为10℃。结果:比阿培南血药浓度在0.2～50μg.mL-1范围内线性关系良好(r=0.9997),最低检测限为0.2μg.mL-1。最低、低、中、高(0.2、0.5、5、50μg.mL-1)浓度血浆样品中的比阿培南的平均提取回收率分别为105.8%、91.5%、86.84%、97.00%,内标的平均回收率为97.04%;4种浓度的日内、日间RSD均<15%,均满足生物样品测定要求。结论:本方法简便、准确、快速、灵敏、专属性强、重现性高,可用于比阿培南人体药动研究。
OBJECTIVE： To establish an HPLC method for the determination of biapenem in human plasma. METHODS： Sample separation was conducted on Hypersil ODS chromatographic column; the mobile phase consisted of acetonitrile- 0.1 mol·L^-1 sodium acetate buffer solution （2 ： 98,pH 4.38） at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at ;300 nm; the column temperature was kept at 25℃ ;the sample size was 20μ and the sample temperature was kept at 10℃. RESULTS： Biapenem showed a good linear relation in the range of 0.2-50μg·mL^-1, with a lowest detectable limit of 0.2μg·mL^-1. The average recovery rates of biapenem at 4 different concentrations （0.2, 0.5, 5, 50μg·mL^-1） were 105.8%, 91.5%, 86.84% and 97.00%, respectively, and the average recovery rate of internal standard was 97.04%. Both the intra - day and inter- day RSD of the 4 concentrations of biapenem were less than 15%, all met the criteria for the determination of biological samples. CONCLUSION： The HPLC method is simple, accurate, rapid, sensitive, specific and reproducible, and it is applicable for the pharmacokinetic study of biapenem in human plasma.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第32期2517-2519,共3页
China Pharmacy
