摘要
将杏原生质体在５％ＤＭＳＯ＋０．５ｍｏｌ／Ｌ山梨醇的冰冻保护剂中以１℃／ｍｉｎ的降温速度降至－４０℃，停留２ｈ后投入液氮保存，４８ｈ后在４０℃水浴中化冻，结果表明，不同品种和不同供体材料的原生质体超低温保存的效果不同。‘龙王帽’杏悬浮培养物分离的原生质体超低温保存后的成活率可达４０％。保存后成活的原生质体分裂频率和植板率均有所提高。
Apricot( Armeniaca vulgaris Lam.)protoplasts were put into cryopretectant(5%DMSO+0.5mol/L sorbitol),cooled at a rate of 1℃/min from 0℃ to -40℃,kept at -40℃ for 2 hours,put into liquid nitrogen and then thawed in 40℃ water bath.The effects of protoplasts cryopreservation were different due to the difference of cultivars and donor materials.The survival rate of protoptasts isolated from“Longwangmao”suspension cultures after cryopreservation could amount to 40%.Higher division and plating frequency was obtained when the survival protoplasts cultured after cryopreservation.
出处
《园艺学报》
CAS
CSCD
北大核心
1998年第4期329-332,共4页
Acta Horticulturae Sinica
基金
国家自然科学基金
关键词
杏
原生质体
超低温保存
Apricot( Armeniaca vulgaris Lam.)
Protoplast
Cryopreservation
