摘要
应用ＰＣＲ方法对ＰＲＶ感染细胞及自然发病猪不同组织样品进行检测，感染细胞毒最低检出量为１０５个ＴＣＩＤ５０病料组织样品最低取样为０１ｍｇ时，仍能得到阳性结果其敏感性显著高于微量血清中和试验。ＰＲＶ在自然发病猪体内分布很广，脑、三叉神经节（三叉Ｎ节）、嗅球、扁桃体、心脏、肝、脾、肺、肾均有ＰＲＶ的存在，ＰＲＶ检出率最高的组织为三叉神经节，其检出率为１００％。其它对照病毒及传代细胞ＰＣＲ反应产物电泳结果均为阴性。
Polymerase chain reaction(PCR) was carried out to amplify DNA fragment of pseudorabies virus from virus infectes cells and naturally infected pigs.The results indicated that 10 5 TCID 50 PRV contents or 0.1mg samples of pigstissues could be detected the PCR.The PCR sensitivity was higher than serum neutralization test.Pseudorabies virus was widely distributed in naturally infected pigstissues including brain,trigeminal ganglion,tonsil,olfactory bulbs,heart,liver,spleen,lung and kidney.The rate of PRV positive detection in trigeminal ganglion was the highest in all examined tissues and reached 100%. The control samples of OPFV,IBRV,PVV,BHK 21 were negative by PCR.
关键词
多聚酶链反应
检测
伪狂犬病毒
Polymerase chain reaction Detection Pseudorabies virus
