摘要
目的探讨儿茶酚胺氧位甲基转移酶(COMT)基因Val158Met的错义突变多态性与老年心肌梗死的关系。方法采用聚合酶链反应限制性内切酶片段长度多态性技术,检测159例老年心肌梗死患者(病例组)及180例健康体检者(对照组)的COMT基因多态性位点频率,分析突变基因型与老年心肌梗死的相关性。结果病例组COMT基因GG、GA和AA型频率分别为66.04%、30.82%和3.14%,对照组分别为55.00%、41.67%和3.33%,两组比较,差异无统计学意义(P>0.05),病例组和对照组GA和AA合并后基因频率分别为33.96%和45.00%,差异有统计学意义(P<0.05),与对照组比较,突变的基因型(GA和AA)在病例组的相对危险性为0.629(95%CI:0.405～0.976,P>0.05)。结论COMT基因Val158Met多态性可能与老年心肌梗死的发生相关。突变基因型可能降低老年心肌梗死的发病风险。
Objective To explore the relationship of Val158Met polymorphism of catechol-O-meth-yltransferase（COMT） gene with myocardial infarction in old people. Methods 159 old patients with acute or secondary myocardial infarction（MI） and 180 normal old people were recruited. The two groups were matched for age, sex, alcohol and cigarette consuming status. All participants were genotyped for COMT at the Val158Met locus using PCR-based restriction fragment length polymorphism method. Results The frequencies of GG,GA and AA genotypes were respectively 66.04%,30.82% and 3.14 % in MI group,and 55.00%,41.67 % and 3.33% in normal control group （P ~ 0.05）. The frequencies of mutant GA＋AA genotypes in MI group were significantly lower than those in normal group（33. 96% vs 45.00%, P〈 0.05）. Conclusion The COMT Val158Met polymorphism is associated with the prevalence of MI among old people. The mutant genotype may decrease the risk of MI.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2009年第11期847-849,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
关键词
心肌梗死
儿茶酚胺类
突变
多态性
限制性片段长度
基因频率
myocardial infarction
catecholamines
mutation
polymorphism, restriction fragment length
gene frequency
