摘要
目的：建立全血中铅的直接快速石墨炉原子吸收光谱分析方法。方法：石墨炉原子吸收光谱仪经优化条件后以硝酸和TritonX-100作为样品稀释液,用磷酸二氢铵作化学改进剂,横向加热塞曼扣除背景,石墨炉原子吸收光谱法直接自动进样测定。结果：分析标准牛血GBW（E）090033、090035中铅的含量,测定结果与标准值相吻合,11次空白测量检出限为1.29μg/L,特征浓度为0.33μg/L/1%A,样品最低检出质量浓度为2.13μg/L,线性范围为0.00~50.0μg/L,相对标准偏差（RSD）为0.043%~0.67%。样品连续6 d测定的相对标准偏差（RSD）为1.23%~6.54%,加标回收率为98.6%~104.6%。结论：本法灵敏度高,重复性好,结果准确可靠,基底干扰效应小,自动化程度高,操作简便。
Objective：To establish the method of analysising the lead in the whole blood by graphite furnace atomic absorption spectrometry（AAS） directly and rapidly.Methods：The atomic absorption spectrometer was optimized,Nitric acid and TritonX-100 dealed with the sample dilution,Ammonium dihydrogen phosphate（ADP） acted as the basal body improver,then transversal heated the Zee-man and deduct the background,the sample was automatic sampling and the lead was detected by AAS.Results：Analying the contents of lead in standard ox blood GBW（E） 090033,090035 coincided with the certified value.Sample introduction 1ul,the lowest detected mass concentration was 2.13 μg/L,the linear range was 0.00~50.0 μg/L.the relative standard deviation（RSD） was 0.043%~0.67%,the sample was dected continuously in six days and the relative standard deviation was 1.23%~6.54%,the recovery rate of adding standard ox blood was 98.6%~104.6%.Conclusion：This methed has higher sensitivity,better reproducibility and the result is exact.The interference of basal body is fewer and the operation is convenient.
出处
《中国卫生检验杂志》
CAS
2009年第11期2542-2544,共3页
Chinese Journal of Health Laboratory Technology
关键词
石墨炉原子吸收光谱法
全血
铅
Graphite furnace atomic absorption spectrometry
GFAAS
Whole blood
Lead
