摘要
目的:建立一种快速检测患者血液标本中恙虫病东方体的方法,用于恙虫病的早期实验室诊断。方法:收集患者血液标本,提取全基因组DNA;根据恙虫病东方体56 kD蛋白编码基因特异基因引物,采用巢式PCR方法对其进行检测,扩增的基因片段进行序列分析。结果:132份标本中,34份得到预期片段大小的PCR扩增产物,随机选取3份扩增产物TA克隆后测序分析,证实PCR扩增产物为恙虫病东方体基因片段。结论:该方法灵敏、特异、快速,可直接用于患者血液标本的恙虫病东方体特异性基因的检测,可为恙虫病东方体感染的早期诊断提供实验室依据。
Objective：To develop a rapid method to detect the presence of Orientia sutsugamushi in the blood samples from suspect patients infected with O.sutsugamushi.Methods：The whole bacterial genomic DNA was extracted from the patient blood samples.The primer pairs used for the nested PCR were designed on the basis of the nucleotide sequence of the gene that encodes the 56-kDa antigen.The PCR productions were sequenced.Results：34 of 132 samples were predictive gene segments and 3 PCR products were sequenced by TA cloning.The sequence analysis confirmed that the gene segments were of O.sutsugamushi.Conclusion：It is evident that this assay is a sensitive,specific and rapid method for the detection of O.tsutsugamushi in blood samples from suspect patient and it can be used for the early diagnosis of O.tsutsugamushi infections.
出处
《中国卫生检验杂志》
CAS
2009年第11期2524-2525,共2页
Chinese Journal of Health Laboratory Technology
