摘要
目的观察非小细胞肺癌气虚血瘀证患者外周血中肿瘤转移相关因子的表达情况。方法随机抽取ⅡB～Ⅲ期非小细胞肺癌患者80例,将入选患者,严格按照中医气虚血瘀证诊断标准,分别归入气虚血瘀证组(40例)和非气虚血瘀证组(40例);采用酶联免疫法(ELISA)检测血清血管内皮生长因子(VEGF)、内皮抑素(Endostatin)及可溶性细胞间黏附分子-1(sICAM-1)含量;采用流式细胞仪技术检测外周血黏附分子CD44(CD44)的表达。结果气虚血瘀证组患者的外周血CD44(136.65±29.60)、血清VEGF[(1002.78±312.08)ng/L]、Endostatin[(120.88±20.00)μg/L]、sICAM-1[(531.78±213.37)μg/L]表达均高于非气虚血瘀证组[分别为98.46±20.64、(653.18±318.99)ng/L、(98.29±23.92)μg/L及(409.36±167.65)μg/L],两组比较,差异均有统计学意义(P<0.01)。结论非小细胞肺癌气虚血瘀证与肿瘤转移相关因子存在客观的内在联系;外周血CD44、血清VEGF、Endostatin、sICAM-1的检测可作为气虚血瘀证的微观辨证依据。
Objective To observe the expressions of tumor metastasis related factors（TMRF）in peripheral blood of patients with non-small cell lung cancer（NSCLC）for exploring the molecular mechanism for genesis of qi-deficiency and blood stasis（QDBS）syndrome in patients.Methods Eighty selected NSCLC patients of stage ⅡB/Ⅲ were differentiated into the QDBS group and the non-QDBS group according to the Chinese medicine syndrome differentiation criteria,40 in each group.The serum levels of vascular endothelial growth factor（VEGF）,endostatin（ES）and soluble intercellular adhesive molecule-1（sICAM-1）in patients were detected by ELISA,and the expression of adhesive molecule CD44 in peripheral blood was determined using flow cytometry.Results Serum levels of VEGF（1002.78±312.08 ng/L）,ES（120.88±20.00 μg/L）,sICAM-1（531.78±213.37 μg/L）and CD44（136.65±29.60）were significantly higher in patients of QDBS group than in patients of non-QDBS group（653.18±318.99 ng/L,98.29±23.92 μg/L,409.36±167.65 μg/L and 98.46±20.64,respectively,P〈0.01）.Conclusion Objective inner links are found between the QDBS syndrome and TMRF in NSCLC patients;serum levels of VEGF,ES,sICAM-1 and CD44 can be served as the microcosmic basis for QDBS syndrome differentiation.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2009年第10期886-888,共3页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
气虚血瘀证
非小细胞肺癌
肿瘤转移相关因子
qi-deficiency and blood stasis syndrome
non-small cell lung cancer
tumor metastasis related factors
