摘要
根据已发表的麦族植物Psy基因序列的保守区设计引物Psy02,克隆小麦Psy基因(片段)。结果表明,Psy02引物的扩增产物出现2种带型:196bp和233bp,序列分析表明两条特异条带涵盖了小麦Psy基因第2外显子全部序列,相差的37bp为Psy基因第2内含子中的一段插入序列,可反映不同黄色素含量(YPC),属小麦Psy基因的等位变异。验证试验表明,248份小麦微核心种质中有153份材料(占样品数的65.7%)扩增出196bp条带,群体内YPC均值7.314mgkg-1,属高YPC范畴;另有95份材料(占样品总数的38.3%)扩增出233bp条带,群体内YPC均值为5.207mgkg-1,属低YPC范畴,方差分析表明二者YPC差异达1%极显著水平差异,说明上述37bp的插入序列是导致小麦品种间YPC产生差异的原因之一,因此该引物扩增的Psy基因对小麦YPC具有显著影响,引物Psy02是对小麦YPC进行分子鉴定的重要标记。
Primer Psy02 was designed according to conservative areas of Psy gene sequence of Ttiticum, and partial sequence of Psy gene of wheat was cloned. The results showed that, two different PCR products were amplified with 196 bp and 233 bp, both of which covered whole sequence of the second exon of wheat Psy gene, and the 37 bp difference between two bands was an insertion sequence of the second exon of Psy gene, which display different YPC in wheat. Verification test showed, that among 248 wheat micro core collections, 153 samples amplified 196 bp band while the rest samples amplified 233 bp band which accounted for 61.7% and 38.3% respectively. The Y-PC average value of 153 samples was 7.314 mg kg^-1 which belong to high YPC rang and that of 95 smaples was 5.207 mg kg^-1 which belong to low YPC rang. Variance analysis showed that the YPC difference reached 1% significant level, which proved the 37 bp insertion sequence was one of the reasons of different YPC in cultivars, in a word, the YPC of wheat was significantly affected by the Psy gene and, primer Psy02 was an important molecular marker to identifying YPC.
出处
《云南植物研究》
CAS
CSCD
北大核心
2009年第5期408-414,共7页
Acta Botanica Yunnanica
基金
国家科技支撑计划(2006BAD01A02)
公益性行业(农业)科研支项(nyhyzx07-002)
农业部"引进国际先进农业科学技术"项目(2006-G2)
关键词
小麦
微核心种质
研基因
等位变异
Wheat
Micro-Core collections
Psy gene
Allelic variation
