摘要
以寄生在金鱼Carassius auratus鳃部的中型指环虫Dactylogyrus intermedius为指示寄生虫,采用活体感染、活体杀虫的方法,研究了用不同溶剂（石油醚（沸程60-90℃）、氯仿、乙酸乙酯、乙醇和水）分别对盾叶薯蓣Dioscorea zingiberensis进行提取,其5种提取物的杀虫活性。结果表明,盾叶薯蓣的杀虫活性部位是乙醇提取物。将乙醇提取物与甲苯咪唑、伊维菌素、阿维菌素和吡喹酮4种原料药的杀虫效果进行比较,结果表明：盾叶薯蓣乙醇提取物的最高杀虫浓度为17.0 mg/L,平均最高杀虫率为99.37%;其次是甲苯咪唑和吡喹酮,最高杀虫浓度分别为3.4、6.2 mg/L时,平均最高杀虫率分别为99.01%和95.39%;而伊维菌素、阿维菌素对中型指环虫无明显的杀灭作用。盾叶薯蓣乙醇提取物对斑马鱼Brachydanio rerio的24、48、96 h的半致死浓度（LC50）分别为53.47、34.05、19.34 mg/L。
The parasiticidal effect of ethanol extracts from a plant Dioscorea zingiberensis on dactylogyrid Dactylogyrus intermedius found in goldfish （ Carassius auratus） was studied to search for parasiticidal substances by standard laboratory procedures compared with the other four drugs mebendazole, abamectin, ivermectin and praziquantel. The bioassay -oriented procedure showed that the active compounds concentrated in the alcoholic extract, with LC50 value of 53.47 mg/L（24 h） ,34.05 mg/L（48 h）, and 19.34 mg/L（96 h）. The anthelmintic efficacy of the alcoholic extract was found to be 99.55% with the concentration of 17.0 mg/L （48 h）. For mebendazole,99.01% of the dactylogyrid were killed with 3.4 mg/L （48 h） drugs, and 95.39% killed by praziquantel at a rate of 6.2 mg/L. It is suggested that the ethanol extracts of Dioscorea zingiberensis be a promising and effective Chinese herbal medicine to control dactylogyrids.
出处
《大连水产学院学报》
CSCD
北大核心
2009年第5期393-399,共7页
Journal of Dalian Fisheries University
基金
西安市科技攻关项目(GG06114)
陕西省山川秀美办公室--世行贷款项目区专项
西北农林科技大学科研专项(07ZR006)
关键词
盾叶薯蓣
指环虫
活性部位
药效
半致死浓度
Dioscorea zingiberensis
Dactylogyrus intermedius
anthelmintic activity
anthelmintic efficacy
LC50
