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人脐静脉内皮细胞培养方法的改进及其优势 被引量：5

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摘要 目的改进血管内皮细胞培养方法。方法用胰酶消化、分离脐静脉内皮细胞,用含20%人AB血清的RPMI1640完全培养液,于37℃、5%CO2条件下培养细胞,以1∶2传代。以传统的20%胎牛血清+基础培养液培养方法(对照组1)和20%胎牛血清+生长因子+基础培养液培养方法(对照组2)作为对照,比较三种方法对细胞活力、培养细胞数量、实验费用等方面的影响。结果以改良方法培养的脐静脉内皮细胞,呈典型内皮细胞外观,特征性地表达内皮细胞标志,改良法培养的细胞数量和细胞活力明显优于对照组1,所需实验费用明显少于其他两组。结论以改良法培养血管内皮细胞,更加简便、经济,可收获大量细胞,可满足大规模内皮细胞实验研究。
作者 牛红心 吕文成 阎晓
机构地区 南方医科大学珠江医院肾内科 南方医科大学南方医院肿瘤科生物治疗中心
出处 《广东医学》 CAS CSCD 北大核心 2009年第11期1621-1623,共3页 Guangdong Medical Journal
关键词 人脐静脉内皮细胞 细胞培养 方法改进
分类号 R563.1 [医药卫生—呼吸系统] R543 [医药卫生—心血管疾病]
  • 相关文献

参考文献7

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二级参考文献3

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共引文献5

同被引文献38

  • 1PENG Kan-fu WU Xiong-fei ZHAO Hong-wen SUN Yan.Advanced oxidation protein products induce monocyte chemoattractant protein-1 expression via p38 mitogen-activated protein kinase activation in rat vascular smooth muscle cells[J].Chinese Medical Journal,2006(13):1088-1093. 被引量：10
  • 2刘宽芝,吕海莉,王伟超,靳陶然,温进坤,韩梅.罗格列酮对2型糖尿病大鼠大血管病变的防治作用及其分子机制[J].中国动脉硬化杂志,2006,14(2):93-96. 被引量：6
  • 3邱雅慧.血管内皮细胞的功能以及损伤修复与动脉粥样硬化[J].中国组织工程研究与临床康复,2007,11(10):1927-1929. 被引量：79
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引证文献5

二级引证文献3

广东医学
2009年 第11期

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