摘要
目的建立同时检测Ｍｙｃ基因３个成员Ｌｍｙｃ、Ｎｍｙｃ及Ｃｍｙｃ异常扩增的简便方法。方法采用聚合酶链反应（ＰＣＲ）技术，用一对引物同时扩增Ｍｙｃ基因３个成员第２外显子中的高度保守区，扩增产物经非变性聚丙烯酰胺凝胶电泳及激光扫描。结果此法可以检测Ｍｙｃ基因家族中３个成员的扩增情况。经检测，正常组织细胞没有Ｍｙｃ基因扩增，３２例喉癌组织中４７％有Ｌｍｙｃ和Ｃｍｙｃ扩增，４１％有Ｎｍｙｃ扩增，与正常比差异均有显著意义（χ２＝６．７６４，７．６０９，５．９６１；Ｐ均＜００５）。Ｃｍｙｃ扩增率与用ＰＣＲ琼脂糖凝胶电泳检测的结果基本相符（χ２＝０．２５４，Ｐ＞００５）。结论此法对检测肿瘤组织中Ｍｙｃ基因３个成员提供了简易、特异、无放射污染，并且适于临床应用的、优于ＰＣＲ琼脂糖凝胶电泳的方法。
Objective To establish a simple method for detecting abnomal amplification of Myc gene family included 3 members(L myc, N myc and C myc) at the same time.Method One pair primer was used to amplify the high conservative region in exon 2 of 3 members of Myc gene by PCR.A PCR PAGE laser scanning technique was applied.Results There was no amplification of Myc gene in the nomal laryngeal tissues.The amplification of abnormal L myc and C myc gene was 47%,and the abnormal N myc gene was 41% in 32 cases of laryngeal cancer.The C myc amplification rate coincided with the result of detecting with PCR agaroe gel electrophoresis ( χ 2= 0.254, P >0.614).Conclusion This method is free from radiactive pollution. It is better than the traditional way of PCR agarose gel eleatrophoresis test and is useful in detecting 3 members of the Myc gene.
关键词
聚合酶链反应
喉肿瘤
MYC基因
Genes, myc Polymerase chain reaction Laryngeal neoplasms
