摘要
植物花粉管质膜类整合素蛋白的免疫印迹检测孙颖徐小冬孙大业（河北师范大学生物系石家庄０５００１６）关键词整合素，花粉管，质膜，免疫印迹ＩＭＭＵＮＯＢＬＯＴＳＯＦＩＮＴＥＧＲＩＮＬＩＫＥＰＲＯＴＥＩＮＳＩＮＰＯＬＬＥＮＴＵＢＥＭＥＭＢＲＡＮＥＯＦＨＥＭ...
Antiserum against human VnR integrin,and αv,β3 integrin subunit cytoplasmic domain in Western blots were applied to determine if integrinlike proteins be present in the pollen tube membrane of Hemerocallis citrina Baroni. The resulst showed that antiβ3 integrin subunit serum could recognize 140 kD and 97 kD bands in SDSPAGE gels under the reducing conditions,while antiserum against VnR and αv integrin could recognize 160 kD and 155 kD bands respectively under the reducing conditions,and also two small bands of higher molecular weight under the nonreducing conditions. Nonimmune serum control could not cross react with any protein bands. The present study suggest that the integrinlike protein,the receptor of vitronectin could exist in the form of αv and β3 subunits in the pollen tube membrane,with its molecular mass quite similar to that of the integrin reported in animal.
关键词
整合素
花粉管
质膜
免疫印迹
Integrin, Pollen tube, Plasma membrane, Immunoblots
