摘要
用微注射法将ＩＡＡ、ＧＡ３、ＫＴ引入开花后３０ｄ的大豆（ＧｌｙｃｉｎｅｍａｘＬ．）完整植株种子的两片子叶之间，７ｄ后取样，分别测定了种皮和子叶中的ＡＢＡ含量、酸性转化酶和ＡＴＰ酶活性，同时分析了子叶中的糖和蛋白质含量的变化。结果表明：１０－６ｍｏｌ／ＬＩＡＡ和１０－６ｍｏｌ／Ｌ的ＫＴ处理使种皮中的ＡＢＡ水平升高，酸性转化酶活性提高，而ＡＴＰ酶活性降低；相反，对照子叶中的ＡＢＡ水平下降，酸性转化酶活性也下降，而ＡＴＰ酶活性显著提高；１０－６ｍｏｌ／ＬＧＡ３的作用正相反，使种皮和子叶中的ＡＢＡ水平均下降，酸性转化酶活性及子叶中的ＡＴＰ酶活性也相应下降，但种皮中的ＡＴＰ酶活性却上升；子叶中的糖和蛋白质含量的变化没有明确的规律。内源ＡＢＡ含量的变化与转化酶和ＡＴＰ酶活性变化呈现一定的相关关系，表明ＡＢＡ参与了调节大豆种子中的同化物积累。对外源激素对大豆种子内源ＡＢＡ水平的影响及其和同化物积累的关系进行了讨论。
Injecting IAA,GA3, and KT into soybean (Glycine max L.) seeds in vivo on intact plant of 30 d after anthesis, the ABA content, invertase and ATPase activity in seed coat and cotyledons were tested respectively. It was revealed that the content of endogenous ABA was correlated with the invertase and ATPase activity. The ABA level increased was 24% and 65% in seed coat by using 10-6 mol/L IAA and 10-6 mol/L KT and decreased 19% and 41% in cotyledons, respectively. Correlatively, ABA stimulated invertase activity and inhibited ATPase activity in seed coat, but the reverse effect of ABA was seen in cotyledons. Treated with 10-6 mol/L GA3, ABA level droped by 42% in seed coat and by 22% in cotyledons. Activities of invertase and ATPase were inhibited in seed coat but stimulated in cotyledons. In studying the changes of sugar and protein in the cotyledons, it was shown that ABA was involved in the assimilate accumulation in soybean seeds. The effects of exogenous hormones on endogenous ABA level and ABA in relation to assimilate accumulation in the cotyledons were discussed.
基金
国家自然科学基金
