摘要
从受三硝基甲苯（TNT）严重污染的土壤和废水中分离筛选到17株可降解TNT的酵母菌和白地霉。其中6株为克鲁斯假丝酵母（Candidakrusei），4株为橡树假丝酵母（C.quercitrusa），一株为无名假丝酵母（C.famata），一株为伯杰汉逊酵母（Hansenulabeijerinckii），一株为亚膜汉逊酵母（H.subpelliculosa），4株为白地霉（Geotrichumcandidum）。对其中6株菌进行了降解TNT的条件实验，发现降解TNT的适宜pH为7，温度为37～40℃。在含75～80mg／LTNT的培养基中，40h内能降解TNT56～74mg／L，去除率达71％～93％。在培养基中加入0．01％～0．05％的葡萄糖作碳源，或加入0.01％～0．1％的酵母膏对6株菌降解TNT的能力略有促进作用。加入铵盐作为氮源则明显抑制这些菌对TNT的降解。
Seventeen strains of yeasts and yeast-like fungi were isolated from soil and waste water samples POlluted by trinitrotoluene (TNT).These strains can degrade 71%-93% TNT when growing in the medium originally containing 70-80mg / L TNT within 40 h. They were identified. Among them, six strains are Candida krusei.four are C. quercitrusa, one is C.famata, one is Hansenula beijerinckii, one is H.subpelliculosa, and four are Geotrichum candidum. Six strains were selected for further studies on the conditions effecting TNT degradation by them. The optimum pH and temperatUre are PH 7 and 37-40℃, respectively. TNT Degradation ability of the strains can be promoted by adding 0.01%-0.05% glucose or 0.01%-0.1% yeast extract into the medium. Addition of 0.05% (NH4)2SO4. or NH4C1 tO the medium can evidently inhibit the degradation of TNT by the strains.
出处
《微生物学报》
CAS
CSCD
北大核心
1998年第4期295-299,共5页
Acta Microbiologica Sinica
关键词
三硝基甲苯
降解
酵母菌
白地霉
Trinitrotoluene (TNT), Degradation, Yeasts, Geotrichum candidum
