摘要
通过ＳｅｐｈａｄｅｘＧ－７５、Ｇ－５０和Ｇ－２５洗脱等步骤，从云南产大蹼铃蟾（Ｂｏｍｂｉｎａｍａｘｉｍａ）皮肤分泌液中分离得到了一种有广泛抗菌活性的多肽，被命名为ＭＳＰ－Ⅶ。用快原子轰击质谱（ｆ．ａ．ｂ．ｍ．ｓ）分析其Ｍ＋／Ｚ＝２６４８，即此活性肽的分子量为２６４７Ｄ。用自动氨基酸分析仪分析，含有２６个氨基酸。抗菌实验结果表明，此多肽对格兰氏阴性和格兰氏阳性细菌以及真菌都有较强的抗菌活性。溶血实验表明此活性肽有很轻微的溶血活性。此外，ＭＳＰ－Ⅶ也可以促进平滑肌收缩和增加微血管的渗透性。
An anti-microbial peptide named MSP-Ⅶ,was isolated from the skin secretion of the toad,Bombina maxima,distributed in Yunnan Province of China,by a three step protocol including Sephadex G-75 and G-50 connected with G-25 two steps of the gel filtration and one reverse-phase high-performance liquid chromatography. Fast atom bombardment mass-spectroscopy of MSP-Ⅶ gave a protonated molecular ion m/z 2648 which matched the theoretical molecular weight predicted from the composition of amino acids. It consists of twenty-six amino acid residues determined by automatic amino acid analyzer. MSP-Ⅶ is a water-soluble peptide. MSP-Ⅶ has a strong anti-microbial activity to bacteria,fungi and yeast with a little hemolysis activity. These characterizations of MSP-Ⅶ make it possible to be used as a clinical anti-microbial medicine. We tested its effect on micro-vessel osmosis ability with Evens blue dye. It was found that MSP-Ⅶ made the permeability of some tissues such as lung,liver,small intestine,to Evens blue dye increase. In particular,this peptide obviously increased permeability of lung. In addition,MSP-Ⅶ displayed an effect on the contraction of smooth muscle,which strengthened the contraction ability of rabbit aorta smooth muscle.
基金
云南省应用基础研究基金
关键词
大蹼铃蟾
抗菌皮肤肽
分离纯化
性质
Bombina maxima ,Anti-microbial skin peptide,Purification,Characterization
