摘要
用16种限制性内切酶研究了银鲫(3N=156～162)、彭泽鲫(3N=162)和缩骨鲫(3N=150)3个三倍体鲫鱼品系及野鲫(2N=100)的线粒体DNA。有6种酶在种系间和种系内产生限制性片段长度多态性(RFLPs),银鲫共存在4种单倍型,彭泽鲫2种,野鲫3种,缩骨鲫1种。彭泽鲫和银鲫拥有相同的常见单倍型,缩骨鲫的单倍型属于野鲫的常见型。根据限制性位点的变异数据,计算了单倍型间的相似性、核苷酸多样性、品系内核苷酸多样性和品系间的遗传距离,确定彭泽鲫属于银鲫的一个地方品系,缩骨鲫属于野鲫的一个地方品系。根据核苷酸的差异,推算出银鲫和野鲫两个亚种的分化大约在11万年前完成。
The mtDNA from three strains of triploid Carassius auratus: C. auratus gibelio(3N= 156～162), C. auratus pengze var. (3N= 162), C. auratus sogu var. (3N=150) and C. auratus auratus (2N= 100) were studied by 16 restriction endonucleases.mtDNA RFLPs were produced inter-or intrastrains for 6 enzymes. Four haplotypeswere found in C. auratus gebilo, 2 in C. auratus pengze var., 3 in C. auratusauratus and 1 in C. auratus sogu var.. C. auratus gibelio and C. auratus pengze var.with the same common haplotype. The haplotype of C. auratus sogu var. is thecommon haplotype in C. auratus auratus. By data of restriction sites variant, thesimilarity and nucleotide diversity among haplotypes, nucleotide diversity of intrastrainand genetic distance among strains were calculated. It is determined that C. auratuspengze var. is a local population of C. auratus gibelio and C. auratus sogu var. is avariant strain of C. auratus auratus. The scientific name of C. auratus pengze var.should be C. auratus gibelo pengze var. Using the nucleotide divergence data betweenthe two subspecies, the divergence time of C. auratus auratus and C. auratus gibeliois estimated to be at least 1.13×10^(-5) years.
基金
淡水生态和生物技术国家重点实验室开放课题基金
淡水鱼类种质资源和生物技术国家重点实验室开放课题基金
关键词
鲫鱼
线粒体DNA
种系鉴定
Carassius auratus
mtDNA
Stock identification
