摘要
多彩色荧光原位杂交是一门新兴的分子细胞遗传学技术，它用几种不同颜色的荧光素单独或混合标记的探针，进行原位杂交，同时检测间期细胞或中期细胞中的几个特异核酸序列，为分析癌症遗传不稳定性提供了一种简便、快速、可靠的方法，并广泛应用于物理图谱绘制、致突变研究、肿瘤病理学和产前诊断．
Multicolor fluorescence in situ hybridization, a new promising molecular cytogenetic technique, permits the simultaneous detection several specific nucleic acid sequences in interphase as well as metaphase by using cohybridization of probes labeled with serveral fluorescent labels or label combinations. It provids a simple, fast, and reliable means to assess genetic instability in cancer. This approach is being applied generally in physical mapping,mutation research, tumor pathology and prenatal dignosis.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第4期333-337,共5页
Progress In Biochemistry and Biophysics
关键词
染色体描绘
启动标记
多彩色FISH
multicolor fluorescence in situ hybridization, chromosome painting, multicolor primed in situ labeling, comparative genomic hybridization
