摘要
目的研究鄂产黄药子对小鼠免疫功能的影响。方法将昆明小鼠随机分为高、中、低3个剂量组,用鄂产黄药子水煎剂进行等容量灌胃,总剂量分别为1000,490和240g·kg-1。另设对照组灌服同体积的生理盐水。连续灌服15d后,用乳酸脱氢酶释放法、碳粒廓清实验进行非特异性免疫检测;用定量溶血分光光度法进行体液免疫检测;用α-醋酸萘酯酶染色法、四甲基偶氮唑蓝法进行细胞免疫检测。结果与对照组相比较,高剂量组能明显降低小鼠巨噬细胞的吞噬作用,而中剂量组能增强小鼠自然杀伤细胞的活性、抗体形成细胞数量、血T淋巴细胞酯酶染色率、T淋巴细胞增殖能力。结论总体上,高剂量鄂产黄药子具有免疫抑制的功能,中剂量具有免疫增强的功能。
OBJECTIVE To study the effects ofHubei Dioscorea bulbifera L.（DB） on immune function in mice. METHODS KM mice were divided randomly into high, medium and low-dose group and control group. The mice in three dose groups were orally administered for 15 d with totally I 000, 490 and 240 g-kg1 body weight of DB respectively, while the mice in control group were treated with same volume of normal saline. Lactate dehydrogenase （LDH） test and carbon particle clearance test were performed to determine the functions of nonspecific immunity. Quantitative hemolysis spectrophotometry （QHS） method was performed to detect the functions of humoral immunity, ct-Naphthy 1.1-acetate esterase （ANAE） staining and methyl thiazolyl tetrazolium （MTT） assays were performed to measure cell-mediated immune function. RESULTS After 15-day administration, compared with the control group, DB markedly reduced the ability of phagocytosis of mononuclear macrophage in the high dose group. DB significantly enhanced the activities of natural killer cells, the antibody quantity of B cells, the quantity and proliferation of spleen T lymphocytes at the medium doses. CONCLUSION Overall, the high dose of DB was able to suppress immune functiOns in mice, while the medium dose can improve immune function.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2009年第17期1309-1311,共3页
Chinese Pharmaceutical Journal
基金
湖北省中药生物技术重点实验室开放基金资助项目(20060204)
关键词
黄药子
小鼠
免疫功能
Dioscorea bulbifera L.
mice
immune function
