期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

人血白蛋白对近端肾小管上皮细胞ACE2表达的影响 被引量：3

The effects of human serum albumin on expression of ACE2 in the proximal tubular cells
暂未订购
导出
摘要 目的:研究人血白蛋白对人近端肾小管上皮细胞(HK-2)血管紧张素转换酶2(ACE2)及血管紧张素转换酶(ACE)表达的影响,探讨白蛋白对肾脏损害的机制。方法:不同浓度人血白蛋白(0、1、5、10、15g/L)分别加入到培养的人近端肾小管上皮细胞培养48h,用RT-PCR法和Western blotting法分别检测HK-2细胞ACE2和ACEmRNA和蛋白表达。结果:正常培养状态下HK-2细胞(空白对照组)存在ACE2mRNA和蛋白表达,加入不同浓度(5-15g/L)的人血白蛋白剂量依赖抑制HK-2细胞ACE2mRNA和蛋白表达(P<0.01)。正常培养状态下HK-2细胞ACEmRNA和蛋白表达水平较低,随着加入白蛋白浓度的增加其表达水平逐渐升高,10g/L白蛋白显著促进了HK-2细胞ACEmRNA和蛋白表达(P<0.05)。结论:在体外培养的HK-2细胞存在ACE及ACE2mRNA和蛋白表达,人血白蛋白可抑制其ACE2表达而促进ACE表达,这可能是白蛋白促肾小管间质损伤及肾小球硬化、间质纤维化的机制之一。
作者 郭汉城 丘昭文 凌毅生 关天俊 周丽华 刘俊兰
机构地区 福建医科大学临床医院暨厦门大学附属中山医院肾内科
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2009年第10期2050-2052,共3页 Chinese Journal of Pathophysiology
关键词 白蛋白类 HK-2细胞 血管紧张素转换酶2 Albumins HK - 2 cells Angiotensin - converting enzyme 2
分类号 R392.2 [医药卫生—免疫学]
  • 相关文献

参考文献9

  • 1孙良忠,岳智慧,陈述枚.白蛋白超载诱导近端肾小管上皮细胞表达α-平滑肌肌动蛋白[J].中国病理生理杂志,2008,24(8):1575-1580. 被引量：5
  • 2Donoghue M,Hsieh F,Baronas E,et al.A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9[J].Circ Res,2000,87(5):E1-E9.
  • 3Wolf G,Wenzel U,Hannken T,et al.Angiotensin II induces p27 (Kip1) expression in renal tubules in vivo:role of reactive oxygen species[J].J Mol Med,2001,79(7):351-352.
  • 4Wong DW,Oudit GY,Reich H,et al.Loss of angiotensin-converting enzyme-2 (Ace2) accelerates diabetic kidney injury[J].Am J Pathol,2007,171(2):438-451.
  • 5Largo R,Gomez-Garre D,Soto K,et al.Angiotensin converting enzyme is upregulated in the proximal tubules of rats with intense proteinuria[J].Hypertension,1999,33(2):732-739.
  • 6Koka V,Huang XR,Chung AC,et al.Angiotensin Ⅱ up-regulates angiotensin Ⅰ-converting enzyme (ACE),but down-regulates ACE2 via the AT1-ERK/p38 MAP kinase pathway[J].Am J Pathol,2008,172(5):1174-1183.
  • 7Donadelli R,Zanchi C,Morigi M,et al.Protein overload induces fractalkine up regulation in proximal tubular cells through NF-κB and p38 MAPK dependent pathways[J].J Am Soc Nephrol,2003,14(10):2436-2446.
  • 8Wakahara S,Konoshita T,Mizuno S,et al.Synergistic expression of angiotensin-converting enzyme (ACE) and ACE2 in human renal tissue and confounding effects of hypertension on the ACE/ACE2 ratio[J].Endocrinology,2007,148(5):2453-2457.
  • 9Wang G,Lai FM,Lai KB,et al.Urinary mRNA expression of ACE and ACE2 in human type 2 diabetic nephropathy[J].Diabetologia,2008,51(6):1062-1067.

二级参考文献17

  • 1张春,朱忠华,刘建社,杨晓,邓安国.结缔组织生长因子在单侧输尿管梗阻大鼠肾脏中的表达及其意义[J].中国病理生理杂志,2007,23(9):1786-1790. 被引量：11
  • 2Iwano M, Plieth D, Danoff TM, et al. Evidence that fibroblasts derive from epithelium during tissue fibrosis [J]. J Clin Invest, 2002, 110(3) : 341 -350.
  • 3Liu Y. Epithelial to mesenchymal transition in renal fibrogenesis : pathologic significance, molecular mechanism, and therapeutic intervention [ J ]. J Am Soc Nephrol, 2004, 15(1): 1-12.
  • 4Fan JM, Ng YY, Hill PA, et al. Transforming growth factor - beta regulates tubular epithelial - myofibroblast transdifferentiation in vitro [ J ]. Kidney Int, 1999, 56 (4) : 1455 - 1467.
  • 5Eddy AA. Proteinuria and interstitial injury [ J ]. Nephrol Dial Transplant, 2004, 19(2): 277-281.
  • 6Brunskill NJ. Albumin signals the coming of age of proteinuric nephropathy [J]. J Am Soc Nephrol, 2004, 15 (2) : 504 -505.
  • 7Kuusniemi AM, Lapatto R, Holmberg C, et al. Kidneys with heavy proteinuria show fibrosis, inflammation, and oxidative stress, but no tubular phenotypic change [ J ].Kidney Int, 2005, 68( 11 ) : 121 - 132.
  • 8Okada H, Danoff TM, Kalluri R, et al. Early role of Fspl in epithelial - mesenchymal transformation [ J ]. Am J Physiol, 1997, 273(4 Pt 2) : F563 - F574.
  • 9Ng YY, Fan JM, Mu W, et al. Glomerular epithelial -myofibroblast transdifferentiation in the evolution of glomerular crescent formation [ J]. Nephrol Dial Transplant, 1999, 14(12) :2860 -2872.
  • 10Rastaldi MP, Ferrario F, Giardino L, et al. Epithelial - mesenchymal transition of tubular epithelial cells in human renal biopsies [J]. Kidney Int, 2002, 62(1): 137- 146.

共引文献4

同被引文献39

  • 1何海伦,陈秀兰,孙彩云,张玉忠.血管紧张素转化酶抑制肽的研究进展[J].中国生物工程杂志,2004,24(9):7-11. 被引量：51
  • 2李怡棠,程桂芳.肾素-血管紧张素系统的新调节分子:ACE2[J].生理科学进展,2006,37(2):179-181. 被引量：4
  • 3杨震,蒋文平.血管紧张素转换酶2的研究进展[J].江苏医药,2007,33(6):606-608. 被引量：2
  • 4Paizis G,Tikellis C,CooperM E,et al. Chronic liver injury in rats and humans upregulates the novel enzyme angiotensin converting enzyme 2[ J]. Gut,2005,54(12) : 1790 - 1796.
  • 5Fagan KA, Morrissey B, Fouty BW, et al. Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension [ J ]. Respir Res,2001,2 ( 5 ) : 306 - 313.
  • 6Ferrario CM. Angiotensin converting enzyme and angiotensin:an evolving story in cardiovascular regulation [J]. Hypertension, 2006,47(3) :515 -521.
  • 7Diez-Freire C, Vazquez J, Correa de Adgunian MF, et al. ACE2 gene transfer attenuates hypertension-linked pathophysiological changes inthe SHR[J]. Physiol Genomics,2006,27 ( 1 ) : 12 - 19.
  • 8Santos RA, Ferreira AJ, Simoes E Silva AC. Recent advances in the angiotensin-converting enzyme 2-angiotensin ( 1 -7 )-Mas axis[ J ]. Exp Physio1,2008,93 ( 5 ) : 519 - 527.
  • 9Fan X, Wang Y, Sun K, et al. Polymorphisms of ACE2 gene are associated with essential hypertension and antihypertensive effects of Captopril in women[ J]. Clin Pharmacol Ther,2007,82 : 187 - 196.
  • 10易著文,陈丹.肾小管上皮细胞损伤在肾小管间质纤维化中的作用[J].实用儿科临床杂志,2007,22(17):1287-1289. 被引量：13

引证文献3

二级引证文献4

中国病理生理杂志
2009年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部