摘要
目的探讨石蜡包埋子宫内膜样腺癌组织中雌激素硫酸转移酶（estrogen sulfotransferase，EST）和甾体硫酸酯酶（steroid sulfatase，STS）的RNA表达。方法使用罗氏产品从30例石蜡包埋子宫内膜组织（对照组和患病组各15例）中提取EST、STS的RNA。结果对照组RNA的表达EST为0．25±0．03，STS为0．08±0．02，STS与EST比值为0．11±0．08；患病组RNA的表达：EST为0．06±0．02，STS为0．24±0．92，STS与EST比值4．40±0．64，患病组与对照组比较，差异有统计学意义（t分别为-8．223、7．345和11．591，均P＜0．05）。结论（1）石蜡包埋组织是子宫内膜样腺癌基础研究的材料来源；（2）在子宫内膜样腺癌中，EST下降，STS和STS与EST比佰升高。
Objective To explore the expression of estrogen sulfotransferase（EST） and steroid sulfatase （STS） in formalin fixed paraffin-embedded endometrioid adenocareinoma samples. MetbodsThe RNA of EST and STS in 30 formalin-fixed paraffin-embedded endometrium samples were extracted using Roehe products, Results The RNA expression of EST and STS were 0, 25±0.03 and 0.08±0, 02 respectively and the STS/EST was 0.11 ±0, 08 in normal endometrium, While in endometrioid adenocarcinoma the RNA expression of EST and STS were 0, 06 ± 0.02 and 0.24 ±0, 92 respeetively and the STS/EST was 4.40±0, 64. There were significant differences between these two groups. Conclusions （1） Formalin-fixed paraffin-embedded tissues could be used to study the endometrioid adenoearcinoma. （2） EST is decreased, STS and STS/EST are increased in human endometrioid adenocareinoma. STS/EST may be related with the prognosis of the endometrioid adenocarcinoma.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2009年第10期840-842,共3页
Chinese Journal of Geriatrics
