摘要
目的观察载体pBBADs-OXM转化双歧杆菌对肥胖小鼠的减肥作用。方法以电穿孔方法将pBBADs-OXM转入双歧杆菌,ELISA及免疫印迹法检测pBBADs-OXM转化长双歧杆菌培养上清及菌体沉淀中OXM的表达。并以此转化双歧杆菌灌饲肥胖小鼠,观察肥胖鼠体质量的变化。结果ELISA、免疫印迹法检测到OXM在pBBADs-OXM转化的双歧杆菌的培养上清及菌体中均有表达。此转化双歧杆菌灌饲的肥胖小鼠体质量显著低于模型组肥胖小鼠(P<0.05)。结论pBBADs-OXM转化的双歧杆菌具有减肥作用。
Objective To observe the effect ofpBBADs-OXM-transformed bifidobacteria on the body weight of obese mice. Methods B. longum was transformed with pBBADs-OXM by electroporation, and arabopyranose-induced oxyntomodulin expression by the bacterium was detected by ELISA. pBBADs-OXM-transformed bifidobacteria was administered orally obese mice on a daily basis with pBBADs-GFP-transformed bifidobacteria as the negative control, and the body weight changes of the mice were observed. Results OXM was detected by ELISA not only in the supernatant but also the precipitant of the transformed bacterial culture. The body weight of the obese mice fed with pBBADs-OXM-transformed bifidobacteria decreased significantly compared with that of the mice in the obese model group （P〈0.05）. Conclusion Administration of pBBADs-OXM-transformed B.longum can reduce the body weight of obese mice.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第9期1796-1798,共3页
Journal of Southern Medical University
基金
国家自然科学基金(30472001)
