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牵张应变作用下人牙周膜细胞形态及钙离子浓度变化的实验研究 被引量：3

The effects of mechanical stretching on morphology and intercellular free calcium concentration of human periodontal ligament cells
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摘要 目的:观察机械牵张应变作用下,人牙周膜细胞(HPDLCs)形态及游离Ca2+浓度的变化。方法:通过原代和传代培养获得性状稳定的人牙周膜细胞,使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载,并加载不同时间,通过流式细胞仪对细胞进行Ca2+浓度的检测。应用SPSS13.0软件对数据进行方差分析。结果:细胞内游离Ca2+浓度随牵张应变时间的延长逐渐升高,至60min时达到该组的峰值(P<0.01);以后随加载时间的延长逐渐下降,120min时降至对照组水平(P>0.05)。结论:牵张应变可以引起细胞形态的改变和细胞内游离Ca2+浓度的变化。 Objective： The purpose of this study is to investigate the effects of mechanical stretching on morphology and concentration of free calcium in the human periodontal ligament ceils （HPDLCs）. Method： Primany human periodontal liga- ment ceils were obtained and passaged. Then the ceils were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain for different time. Free Ca2＋ concentration were examined through Flow cytometry. The data were analyzed by one-way ANOVA using SPSS 13.0 software package. Result： It was significantly higher of the concentration of the free Ca2＋ in ceils which were stretched for 60min than that in the control cells （P 〈 0.01） ： As the stretching time was prolonged, the concentration of the free Ca2＋ decreased to the level of that of control ceils after 120 min stretching. （P 〉 0.05）. Conclu- sion： Stretching force not only influenced morphology but also induced the change of concentration of the free Ca2＋ of cells. Supported by Shanghai Leading Academic Discipline Project.
作者 李隽 胥春 郝轶 张富强
机构地区 上海交通大学医学院附属第九人民医院口腔修复科.上海市口腔医学重点实验室
出处 《临床口腔医学杂志》 2009年第10期582-585,共4页 Journal of Clinical Stomatology
基金 上海市重点(特色)学科建设项目(T0202) 上海市重点学科建设项目(S30206) 上海市科学技术委员会项目(08DZ2271100) 上海市自然科学基金项目(07ZR14070) 上海市科委课题(08411961500) 上海市口腔医学研究所课题(200805)
关键词 人牙周膜细胞 游离钙离子 机械牵张应变 human periodontal ligament cells free calcium ion mechanical stretching
分类号 R780.2 [医药卫生—口腔医学] Q813.1 [生物学—生物工程]
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参考文献8

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同被引文献28

  • 1仲维广,胥春,米红林,张富强.细胞应变装置中硅橡胶膜的应力分析[J].医用生物力学,2007,22(2):121-126. 被引量：15
  • 2Zhong W, Xu C, Zhang F, et al. Cyclic stretching force-induced early apoptosis in human periodontal ligament cells[J]. Oral Dis, 2008,14(3):270-276.
  • 3Xu C, Zhang FQ, Li J, et al. Cyclic stretching influenced Connexin 43 of human periodontal ligament cells[J]. J Dent Res, 2008, 87(Spec Iss B):2812.
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  • 10Zhong W, Xu C, Zhang F, et al. Cyclic stretching force-induced early apoptosis in human periodontal ligament cells [J]. Oral Diseases, 2008, 14 (3) : 270-276.

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临床口腔医学杂志
2009年 第10期

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