摘要
目的探讨记录家猫多焦视网膜电图(mfERG)的方法,分析家猫mfERG的特征及应用价值。方法采用CRT显示器及共焦激光扫描检眼镜(SLO)2种刺激方式分别对10只家猫(20只眼)视网膜进行刺激,记录其mfERG特征。结果CRT显示器刺激见mfERG一阶反应N1波、P1波振幅密度逐渐减小,N1波、P1波振幅密度及峰潜时各区域差异均无统计学意义。SLO显示中心区N1波、P1波振幅密度明显降低,随着离心度的增加,N1波、P1波振幅密度增加;刺激视盘鼻侧区域各环形振幅密度较为平均;刺激视盘颞侧视网膜,随离心度的增大,N1波、P1波振幅密度逐渐减小。结论SLO刺激可提高家猫mfERG的可靠性,其一阶反应呈以视盘颞侧区域为中心的反应尖峰,与人中心凹反应尖峰非常类似。
Objective Muhifucal electroretinography （mfERG） technique has over three different ways in stimulating fashion at present. The study was to investigate the result of mfERG in cats with different stimulating ways and evaluate the feasibility of eat as an experimental model for mfERG research. Methods MfERG was recorded in 10 domestic eats with RETI scan systems （Version 3. 11 ） made in Roland company and Heidelberg confocal laser fundus scanning system（ HRA-FA ＋ ICG） made in Germany. Different stimulating fashions, including CRT monitor and scan laser ophthalmoscope （ SLO ） , were selected to obtain the recording result. The characteristics of mfERG from two types of simulations were analyzed and compared. Results The waves of mfERG of cats from CRT stimulation showed 6 ring zones fi＇om center 0° to 30° according to the eccentrical degree. The mean amplitude density of wave N1 and PI in the first order kernel of mfERG was gradually decreased from 1 ring to 6 rings, and no evidential change was seen in the latency in various rings. There were no significant differences in amplitude density and peak response latency of wave N1 and PI among four quadrants including superior of temporal, superior of nasal, inferior of temporal and inferior of nasal. The waves of mfERG of cats from SLO showed that the amplitude density of wave NI and PI was lower obviously in center zone but enhanced with the increase of eccentrical degree of retina. The amplitude density of each ring was even in cat optic disc nasal area,whereas that of wave N1 and P1 decreased gradually with the increase of eccentrial degree of retina, and no significant change was seen in latency from cat temporal of optic disc area. Conclusion The record method of SLO combined with HRA can avoid the trouble of fixation and improve the reliability of mfERG result. The first order kernel peak response of temple area of optic discs can be recorded from domestic cats,which is similar to the response of human fovea. Cat was a kind of ideal animal model for mfERG research.
出处
《眼科研究》
CAS
CSCD
北大核心
2009年第10期849-853,共5页
Chinese Ophthalmic Research
基金
国家自然科学基金资助(30171172)
关键词
多焦视网膜电图
激光扫描检眼镜
动物模型
multifoeal electroretinogram
scan laser ophthahnoscope
animal model
