摘要
背景与目的:KLF4(Krppel-like factor4)是一种在多种组织中广泛表达的锌指转录因子,在许多不同的生理活动中具有重要作用,研究表明KLF4基因与多种肿瘤的发生发展有关,我们通过检测胃癌组织中KLF4蛋白的表达,以探讨KLF4蛋白与胃癌临床病理间的关系。材料与方法:采用免疫组化SP法,分别检测102例胃癌手术标本的癌组织,癌旁组织及同期收集的正常胃组织标本中KLF4表达的情况。结果:KLF4蛋白表达位于细胞质及细胞核,在癌旁组织或正常胃组织呈强阳性表达,而在胃上皮内瘤变中表达较弱,胃癌组织中KLF4蛋白几乎未见表达。KLF4表达随胃癌分期的变化(I～IV)其表达依次下降(P<0.05)。有淋巴结转移组KLF4蛋白阳性表达率为63.3%,无淋巴结转移组的阳性表达率为78.6%,其差异有统计学意义(P<0.05)。结论:KLF4蛋白在胃癌中表达降低,并与胃癌发生、分期及淋巴结转移有关,可作为胃癌预后的判断指标及潜在的治疗靶点。
BACKGROUND AND AIM：KLF4（Krüppel-like factor 4） is a zinc-finger transcriptional factor expressed in a variety of tissues in humans,and plays an important role in different physiological functions.Studies suggested that KLF4 is associated with the development and progression of various tumors.The study was to investigate the expression of KLF4 in gastric cancer and its correlation with clinicopathology.MATERIALS AND METHODS：The expression of KLF4 was detected in cancer and adjacent tissues of 102 surgical specimens by immunohistochemistry. The normal gastric tissues collected simuhaneously was also examined. RESULTS： KLF4 was expressed in cell nuclei and cytoplasm. Strong expression was detected in adjacent tissue and normal gastric mucosa. Weak expression was detected in intraepithelial neoplasia and negative expression in gastric cancer. There is statistical significance among groups（P 〈 0.05） . The KLF4 expression in gastric cancer decreased with the progression from stage I to stage IV（ P 〈 0.05） . The expressions of KLF4 in groups with and without lymph node metastasis were 63.3% and 78.6%, respectively. There was statistical significance between the two groups（P 〈 0.05）. CONCLUSION： The expression of KLF4 decreased in gastric cancer and showed correlation with gastric carcinogenesis, staging and lymph node metastasis. KLF4 might be the prognostic marker and potential therapeutic target for gastric cancer.
出处
《癌变·畸变·突变》
CAS
CSCD
2009年第5期388-391,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
重庆市卫生局医学科学技术研究项目(2008-2-49)
关键词
胃癌
KLF4
免疫组化
治疗
gastric cancer
KLF4
immunohistochemistry
therapeutics
