摘要
目的：研究不同fimA基因型牙龈卟啉单胞菌（Porphyromonas gingivalis,Pg）对人牙龈成纤维细胞表达IL-8的影响,探讨fimA基因型与Pg致病力差异之间的关系。方法：PgATCC 33277（Ⅰ型）,WCSP115（Ⅱ型）,WCSP1.5（Ⅲ型）,W83（Ⅳ型）分别与牙龈成纤维细胞在标准条件下共同孵育,于孵育后1、3、6、12 h收集细胞和上清,应用实时荧光定量RT-PCR和ELISA法分别检测牙龈成纤维细胞IL-8 mRNA和蛋白的动态表达。结果：与对照组比较,各fimA型Pg对牙龈成纤维细胞IL-8mRNA和蛋白的表达均有明显的促进作用（P＜0.01）;其中ⅡfimA型组IL-8 mRNA和蛋白的表达量明显高于其它fimA型组,不同时间点IL-8 mRNA相对表达量分别为20.42±2.21～103.01±12.50,蛋白分泌水平为（137.46±4.97～323.24±13.74）pg/ml;而ⅢfimA型Pg组的表达水平弱于其它fimA型组,IL-8 mRNA相对表达量为3.61±0.39～12.88±1.56,蛋白分泌水平为（44.83±3.68～189.19±87.34）pg／ml，差异有统计学意义（P〈0．05）。结论：Pg可促进牙龈成纤维细胞IL-8mRNA和蛋白的表达，且各fimA型Pg的促进作用有所差异。提示：fimA基因型的不同可能是pg的致病力差异的基因基础。
Objective：To investigate the mechanism of IL-8 expressions in human gingival fibroblasts（HGFs） regulated by Porphyromonas gingivalis（Pg） with different fimA genotypes.Methods：Pg ATCC 33277（typeⅠ）,WCSP115（type Ⅱ）,WCSP1.5（type Ⅲ）,W83（type Ⅳ） were assessed for their inductions of IL-8 expression in HGFs.IL-8 mRNA levels of HGFs were determined by Real-time RT-PCR and protein levels in culture supernatant were determined by ELISA at different time intervals（1,3,6 and 12 h） following continuous co-culture of bacteria with HGFs. Results： The IL-8 mRNA and protein expressions of HGFs co-cultured with Pgwere significantly increased compared with the negative control group （P 〈 0. 01 ）. The group of type Ⅱ showed greater up-regulation than otherfimA genotypes on the mRNA and protein expressions of IL-8, mRNA 20.42 ±2.21 ～ 103.01 ± 12.50 and protein （ 137.46 ±4.97 ～ 323.24 ±13.74） pg/ml for different time periods, while the group of type 11I was weaker than otherfimA genotypes, the level of IL-8 mRNA 3.61 ± 0.39 ～12.88 ± 1.56 and protein （44.83 ± 3.68 ～ 189.19 ±87.34 ） pg/ml （P 〈 0.05 ）. Conclusion： Pg could induce HGF to over-express IL-8, fimA genotypes of Pg may be related with this pathogenicity, which might indicatefimA genotype is associated with pathogenesis of Pg.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2009年第5期613-616,共4页
Journal of Practical Stomatology
基金
国家自然科学基金资助项目(编号:30471890)
