摘要
以鱼腥草叶片为外植体,比较不同植物生长调节剂、抗褐化剂和光照条件对愈伤诱导率及褐化率的影响。结果表明,MS+6-BA 0.5 mg.L-1+2,4-D 1.0 mg.L-1有利于鱼腥草愈伤组织的诱导;抗坏血酸(Vc)、聚乙烯吡哆烷酮(PVP)对抑制外植体褐变均有作用,其中,PVP效果最佳,最适浓度为2 g.L-1。光照条件对鱼腥草褐变的影响较大,培养初期暗培养,能显著地抑制外植体的褐变。
In this paper,we used the leaves of Houttuynia cordata Thunb as experiment material to compare the effect of different hormones,anti-browning agents and light on callus inducing and browning.The results indicated that MS addition with 6-BA 0.5 mg·L^-1 and 2,4-D 1.0 mg·L^-1 was the optimal induction medium.VC and PVP can restrained browning of the explants.The effect of PVP was the best,its optimal concentration was 2 g·L^-1.Light had obvious effect on browning of Houttuynia cordata Thunb.Browning of the explants could be restrained obviously without light at the early time of tissue culture.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2009年第4期585-588,共4页
Journal of Anhui Agricultural University
基金
安徽省高校"十五"优秀人才计划科研项目
安徽省优秀青年基金(06041083)共同资助
关键词
愈伤组织
诱导
褐化
callus
induction
browning
