摘要
对表现叶片黄化的玉兰植株,利用植原体16SrRNA基因通用引物进行巢式PCR检测,得到1.4kb的特异片段,将此片段克隆后进行序列测定、分析及构建系统关系树.结果表明,该片段与16SrI组中的各植原体同源率均达到99%以上,而与其他组的植原体16SrDNA序列的同源率均低于97%,认为该植原体株系为翠菊黄化植原体组中的成员之一.
Phytoplasma universal primers for 16S rRNA gene was used to detect phytoplasma associated with Magnolia leaf showing yellows symptom. A 1.4 kb DNA fragment was amplified by nested-PCR from the total DNA of diseased Magnolia samples. After cloning and nucleotide sequencing of the amplified fragment, a phylogenetic tree based on 16S rDNA sequences was constructed. The results showed that the strain shared identities of more than 99% with other members in 16SrI group, but obviously under 97.0% with other groups. So it is clear that this phytoplasma strain is one of the members of Aster yellows phytoplasma group.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第5期533-536,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家质量监督检验疫总局行业公益项目(200810517)
关键词
玉兰黄化病
分子检测
系统进化
Magnolia denudata yellows
molecular
phylogenetic
