摘要
目的探讨脾虚型(缓解期)哮喘儿童外周血Th细胞和T细胞亚群的状态及意义。方法设正常对照组(n=38),脾虚型(缓解期)哮喘组(n=17),采用荧光单细胞内染色法检测外周血Th1、Th2细胞活性,采用单克隆抗体间接免疫荧光法检测T细胞亚群,统计检测结果并进行统计分析。结果脾虚型缓解期哮喘组与对照组比较,Th1与对照组比较无显著性差异(P>0.05),Th2明显升高,Th1/Th2明显降低(P<0.01);CD+3无显著性差异(P>0.05),CD+4、CD+4/CD+8均明显降低(P均<0.01),CD8+明显升高(P<0.01);结论脾虚型缓解期哮喘儿童存在Th1/Th2细胞偏移和T细胞亚群的紊乱,偏移的原因主要为Th2细胞活性明显升高所致。
Objective To investigate the state and significance of Th cell and T cell subgroups in peripheral blood in children with asthma of spleen deficiency type（ recovery stage）. Methods Normal control group （ n = 38 ） and asthma group of spleen deficiency type （ recovery stage） （ n = 17 ） were designed. Fluo-rescence mononuclearcytes coloring was used to detect the activity of Thl and Th2 cells in peripheral blood. Monoclonal antibody indirect immunofluorescence was applied to detect T cell subgroup. The statistical analysis was conducted for the detected results. Results Compared with control group, in asthma group of spleen deficiency type（ recovery stage ） , there was no significant difference in Thl （P 〉 0.05 ） , but Th2 increased apparently and Th1/Th2 decreased apparently （ P 〈 0.01 ） ; there was no significant difference in CD3^＋ （ P 〉 0.05 ）, but CD4^＋ and CD^4＋4/CD s decreased apparently （ P 〈 0.01 ）, and CD s increased apparently （ P 〈 0.01 ）. Conclusion The children with asthma of spleen deficiency type （ recovery stage） have the excursion of Th1/Th2 and the disturbance of T cell subgroup. The excursion is mainly induced by the apparent increase of Th2 cell activity.
出处
《世界中西医结合杂志》
2009年第10期727-728,731,共3页
World Journal of Integrated Traditional and Western Medicine
关键词
哮喘
儿童
缓解期
TH细胞
脾虚型
Asthma
Children
Recovery stage
Th cell
Spleen deficiency
