摘要
棒曲霉（ＡｓｐｅｒｇｉｌｕｓｃｌａｖａｔｕｓＤｅｓ）ＨＤ－１１是从绿衣观音土曲中分离得到的，具有较高糖化酶活性的菌株。初步研究结果表明，３０℃下，棒曲霉麸曲培养３０～４０ｈ，其产酶量达到高峰。糖化酶最适作用温度５０～６０℃，最适作用ｐＨ３．０～４．０。糖化酶在４５℃以下，ｐＨ４０～８．０范围内稳定，对淀粉水解程度为７９％，产物为葡萄糖、麦芽糖和少量低聚糖。
spergillus clavatus strain HD-11 was isolated from the Green Kaolinite Koji. When cultured at 30 ℃ for 30 ～40 h in bran medium, its glucoamylase activity got the peak. The optimum pH and temperature for the enzyme were 3～4 and 50～60 ℃ respectively. The enzyme was stable at the temperature below 45 ℃ and the pH varying from 4 to 8. The enzyme can hydrolyse starch into glucose, maltose and small amount of oligosaccharide.
出处
《湖北农学院学报》
1998年第3期250-252,共3页
Journal of Hubei Agricultural College
关键词
棒曲霉
糖化酶
酒曲
spergillus clavatus
glucoamylase
the optimum range of activity
