摘要
目的探讨血小板活化因子乙酰水解酶（PAF-AH）基因Val379Ala位点多态性与脓毒症易感性及预后的关系。方法采用PCR-RFLP技术，分析82例不同程度脓毒症患者及90名健康对照者PAF-AH基因Val379Ala位点的多态性，对含有等位基因多态性的片段进行DNA测序分析。结果全部样本经PCR-RFLP分析后，脓毒症组82例中，仅1例纯合子Val／Val型，24例杂合子Val／Ala型，57例纯合子Ala／Ala型；对照组90例中，2例Val／Val型，29例Val／Ala型，59例Ala／Ala型。Val379Ala基因型和等位基因频率：脓毒症组与对照组比较差异无统计学意义（P〉0.05），存活组、死亡组与对照组比较差异无统计学意义（P〉0.05），轻型脓毒症组、严重脓毒症组与对照组比较差异无统计学意义（P〉0.05）。结论PAF-AH基因Val379Ala位点多态性与脓毒症易感性、预后及病情严重程度可能不相关。
Objective To discuss the allele frequency and genotypic distribution of platelet - activating factor acetylhydrolase （PAF-AH） gene polymorphism in the patients with sepsis. Methods Val379Ala site genotypes were determined in the patients（n=82）and healthy controls（n=90） by means of restriction fragment length polymorphism analysis of polymerase chain reaction products, DNA sequencing was used to detect the PCR product containing allele gene polymorphism. SPSS 13.0 statistical software was used to analyze. Results All the samples by PCR - RFLP analysis of the PAF-AH gene in the Val379Ala site had three kinds of genotypes ： in 82 cases of sepsis group, there were 1 homo- zygous Val/Val type, 24 heterozygous Val/Ala type, 57 homozygous Ala/Ala type; in 90 cases of control group, there were 2 Val/Val type, 29 Val/Ala type, 59 Ala/Ala type. The Va1379Ala allele frequency and genotypic distribution in the patients with sepsis was not significantly different from those in the healthy controls（P〉 0.05） ; no statistically significant difference was observed among the survival group , the death group and the control group （P〉0.05） ; no statistically significant difference was ob- served among the mild sepsis group, severe sepsis group and control group （P〉0.05）. Conclusion No correlations were found between PAF-AH gene Val379Ala polymorphism and sepsis.
出处
《中国急救医学》
CAS
CSCD
北大核心
2009年第11期999-1002,共4页
Chinese Journal of Critical Care Medicine
