摘要
以魔芋葡甘聚糖为原料,通过戊二醛交联作用制备出海绵状的载体,并对脲酶进行固定化。实验研究了脲酶的最佳固定化条件,比较了固定化脲酶与游离脲酶的酶学性质。研究结果表明,载体最优制备条件为:1.5 g魔芋精粉充分溶解于30 mL 0.1 mol/L的NaOH溶液中,经50%0.1 mol/LNaOH的乙醇溶液不溶性处理,5%的戊二醛溶液活化1.5 h;最佳的联酶条件是在4℃下联酶60 min。该固定化脲酶的最适温度为60℃,最适pH为7.5,米氏常数Km为0.001 87。研究表明,载体经冷冻处理后再固定化可以大大提高固定化脲酶的稳定性。
The spongiform konjac glucomannan （KGM） carrier is prepared with KGM and glutaraldehyde, and urease is immobilized on the carrier. The optimum condition for the immobilization of urease is measured and the characterizations of immobilized urease are compared with the free urease. The results show that the optimum condition for KGM carrier preparation is to dissolve 1.5 g refined konjac flour into 30 mL 0. 1 mol/L NaOH, freeze the obtained substance and treat it insolubly using 50% 0. 1mol/L NaOH in ethanol, and activate the carrier with 5% glutaraldehyde for 1.5 h. Reacting the carrier with urease for lh at 4℃ can achieve the highest enzyme activity. For the immobilized urease, the optimum temperature is 60℃, the optimum pH is 7.5, and the Michaelis constant （Kin） is 0. 001 87 mol/L. The results also show that the stability of the carrier can be greatly improved through freezing treatment.
出处
《南京理工大学学报》
EI
CAS
CSCD
北大核心
2009年第5期687-691,共5页
Journal of Nanjing University of Science and Technology
关键词
魔芋葡甘聚糖
戊二醛
脲酶
固定化
海绵状
载体
konjac glucomannan
glutaraldehyde
urease
immobilization
spongiform
carriers
