摘要
目的：动态观察雌二醇（Ｅ２）对单个血管平滑肌细胞内游离钙离子浓度及血管紧张素Ⅱ引致的细胞内钙离子浓度变化的影响，以探讨雌激素对平滑肌细胞内储存钙释放的调节作用。方法：体外培养健康雌性兔胸主动脉中层平滑肌，细胞接种在５７０型粘附式细胞仪专用的培养皿中，加入１０－５、１０－７、１０－９ｍｏｌ／Ｌ３种不同浓度的Ｅ２处理。另用３种不同浓度的Ｅ２预处理细胞１小时后，加入血管紧张素Ⅱ。实验分单纯血管紧张素Ⅱ处理组和血管紧张素Ⅱ＋不同浓度Ｅ２预处理组，用ｆｕｒａ－３法测定细胞内钙离子浓度。结果：３种浓度Ｅ２对平滑肌细胞内游离钙离子浓度的基础水平无影响，但１０－５、１０－７ｍｏｌ／Ｌ的Ｅ２预处理细胞后血管紧张素Ⅱ未能激发细胞内游离钙离子浓度升高。
Objective:In order to investigate the regulating effect of estrogen on the release of cytosolic free calcium in vascular smooth muscle cells (VSMCs),the change of basic or angiotensin Ⅱ(AngⅡ) induced[Ca 2+ ]in single VSMC was determined. Methods:The aortic smooth muscle cells from female rabbits were cultured in vitro for experiments.The cultured cells of 2—4 passages were divided into 3 groups:Estradiol group,different dosage of estradiol(10 -5 ,10 -7 and 10 -9 mol/L ,respectively) was added in the medium;estradiol+AngⅡgroup,pretreated with estradiol as above group for 1h, then AngⅡ(10 -7 mol/L) was added;AngⅡgroup,AngⅡadded alone without any pretreatment.Cytosolic Ca 2+ was measured with fura 3. Results:All the three dosages of estradiol used did not affect the basic [Ca 2+ ]in VSMC,but the release of Ca 2+ induced by AngⅡdisappeared after pretreated with 10 -5 and 10 -7 mol/L estradiol. Conclusion:Estrogen may be involved in the regulation of the change of [Ca 2+ ]in VSMCs induced by AngⅡ.
出处
《中国循环杂志》
CSCD
北大核心
1998年第5期308-310,共3页
Chinese Circulation Journal
