摘要
目的：探讨六亚甲基二乙酰胺（ＨＭＢＡ）联合辐射对人粘液表皮样癌ＭＥＣ－１细胞超微结构的作用特点。方法：实验分４组，对照组细胞未经药物及射线处理；ＨＭＢＡ组细胞经１ｍｍｏｌ／Ｌ的ＨＭＢＡ诱导作用９６ｈ；γ－射线组细胞在乏氧状态下接受７Ｇｙ的６０Ｃｏγ－射线照射；联合应用组细胞按上述方法先经ＨＭＢＡ处理再接受辐射，用扫描电镜和透射电镜观察各组细胞的形态变化。结果：ＨＭＢＡ组细胞表面微绒毛减少，胞浆线粒体增多；γ－射线组细胞微绒毛凝聚，线粒体肿胀、空化；联合应用组细胞损伤明显，微绒毛凝聚、崩解，线粒体粘连、缺嵴、空化和紊乱。结论：ＨＭＢＡ能增强γ－射线对ＭＥＣ－１细胞超微结构的破坏作用。
Objective: To investigate the effects of hexamethlene bisacetamide(HMBA) combined with γ-ray on the ultrastructure of human mucoepidermoid carcinoma MEC-1 cell line. Methods: MEC- 1 cells were treated with 1 mmol/L of HMBA for 96 h, 7 Gy of γ ray under anaerobic condition, or their combination respectively and then cells were observed under the scanning elctron microscopy and the transmission elctron microscopy. Results: Reduction of microvilli on the cell surface and increase of mitochondria in the cytoplasma were observed in HMBA treated cells; microvilli coagulation in γ ray treated; severe destruction of microvilli and mitochondria in HMBA and γ ray treated.Conclusion: HMBA can enhance the destructive effects of γ ray on ultrastructure of MEC-1 cells.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
1998年第4期284-285,共2页
Journal of Practical Stomatology
基金
国家自然科学基金
关键词
六亚甲基
二乙酰胺
粘液表皮样癌
超微结构
Mucoepidermoid carcinoma
Radiation
Hexamethlene bisacetamide
Ultrastructure
