摘要
以雄性奥利亚罗非鱼为试验材料,分别以0、1、3、5和10μg.L-1氯化三丁基锡(TBTCl)通过接触染毒的方式,研究TBTCl对鱼类精巢细胞凋亡的影响。细胞凋亡分析结果表明,在同一染毒时间下,除染毒时间为24和48h,染毒浓度为1μg.L-1处理组与对照组之间细胞凋亡率差异不显著(P>0.05)以外,其余情况下各处理组与对照组之间差异均显著(P<0.05或P<0.01)。各相邻2个浓度处理组之间,3μg.L-1处理组和5μg.L-1处理组之间细胞凋亡率差异均不显著(P>0.05),其余各组之间差异均显著(P<0.05或P<0.01)。随着TBTCl浓度的升高,罗非鱼精巢细胞凋亡率逐渐升高,剂量-效应关系显著。精巢细胞Ca2+-ATP酶活性测定结果表明,在同一染毒时间下,除在染毒时间为24和96 h时,染毒浓度为1μg.L-1处理组与对照组之间差异不显著(P>0.05)以外,其余情况下各处理组与对照组之间差异均显著(P<0.05或P<0.01)。在同一染毒时间下,各处理组之间,除染毒时间为24 h时,染毒浓度1μg.L-1处理组和3μg.L-1处理组之间差异不显著(P>0.05)以外,其余情况下各处理组之间差异显著(P<0.01);随着染毒浓度的升高,Ca2+-ATP酶活性逐渐升高,并且剂量-效应关系显著(P<0.05或P<0.01)。奥利亚罗非鱼精巢细胞凋亡率与精巢Ca2+-ATP酶活性间具有一定的协同关系。
Effect of tributyltin chloride （TBTC1） on apoptosis of spermary cells of male Oreochromis aureus was studied through an experiment,where TBTCI was dissolved in the water the fish were kept in at a dosage of 0,1,3,5 and 10 μg.L^-1 , respectively. Spermaries of the fish were taken after exposure for 24 or 48 hours. Part of the organ was prepared into paraffin section for analysis of apoptosis by terminal deoxynucleotidy transferase mediated dUTP-Biotin nick-end labeling （TUNTEL） ,while the other part was used for study of effect of TBTC1 on activity of Ca^2＋-ATP enzyme in the spermary cell. Results show that apoptosis of spennary cells was induced by TBTCI,and activity of the Ca^2＋-ATP enzyme of spermary cell varied between treatments of dosage of TBTC1 after exposure for a set period of time. Regardless of exposure time, whether 24 or 48 hours and 24 or 96 hours, no significant difference （ P〉0. 05 ） was observed between the 1μg.L^-1 group and the control group in apoptosis and in variation of Ca^2＋ -ATP enzyme activity, respectively. Under the condition of effective dosages, spermary cell apoptosis rate increased with the dosage, showing a close relationship （ P〈0. 05 ） between dos- age and apoptosis rate. Similarly, the activity of Ca^2＋-ATP enzyme also increased along with the dosage of TBTCI （P〈 0. 05 ）. When the dosage of the TBTC1 increased,the apoptosis rate and the activity of Ca^2＋-ATP enzyme of spermary cells increased synergetically.
出处
《生态与农村环境学报》
CAS
CSSCI
CSCD
北大核心
2009年第4期83-87,共5页
Journal of Ecology and Rural Environment
基金
农业部渔业生态环境重点开放实验室开放基金(2005-6)
广东省渔业生态环境重点实验室开放基金(2005-6)
