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CD4^+CD25^+调节性T细胞在α-GalCer预防T1D中的作用机制探讨

The Role of CD4^+CD25^+T Regulatory Cells in Preventing the Development of T1D in α-Gal Cer Cer-treated NOD Mice
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摘要 目的:研究CD4+CD25+调节性T细胞在α-GalCer预防NOD小鼠T1D中的作用机制。方法:流式细胞仪分析反复注射α-GalCer的NOD小鼠胰腺引流淋巴结(PLN)CD4+CD25+T细胞频率,CD4+CD25+T细胞中FoxP3+细胞的比例,CD4+CD25+FoxP3+T细胞的FoxP3平均荧光强度(MFI);检测CD4+CD25+调节性T细胞的抑制功能。用Anti-CD25阻断CD4+CD25+调节性T细胞的功能。结果:α-GalCer处理过的小鼠的CD4+CD25+T细胞中FoxP3+细胞的比例增加,CD4+CD25+FoxP3+T细胞的FoxP3平均荧光强度(MFI)增强,CD4+CD25+Treg细胞的抑制功能增强。采用CD4+CD25+调节性T细胞功能阻断剂后,α-GalCer不能预防T1D的发生。结论:CD4+CD25+调节性T细胞在α-GalCer预防NOD小鼠T1D中数量增加、功能增强,且是必需的。 Objective To investigate the role that CD4＋CD25＋T regulatory（CD4＋CD25＋Treg） cells played in preventing the development of typeⅠ diabetes（T1D） in α-GalCer-treated NOD mice.Methods The frequency of CD4^＋CD25^＋T cell from pancreatic lymph node（PLN） was analysed,the percentages of CD4^＋CD25^＋T cells expressing FoxP3 were observed,mean fluorescent intensity（MFI） of FoxP3 in CD4^＋CD25^＋ FoxP3^＋T cells was assayed in vitro.In vivo suppression experiment of CD4^＋CD25^＋Treg cells with anti-CD25^＋ was performed.Results Both the percentages of CD4^＋ CD25^＋T cells experssing FoxP3 and the mean fluorescent intensity（MFI） of FoxP3 in CD4^＋CD25^＋ FoxP3^＋T cells were increased after α-GalCer treatment.With in vitro suppression assay,CD4^＋CD25^＋Treg cells showed increased ability to suppress the proliferation of CD4^＋CD25^＋T cells.In vivo suppression experiment showed that α-GalCer could not prevent the development of T1D in NOD mice after suppression of CD4^＋CD25^＋Treg cells.Conclusion CD4^＋CD25^＋Treg cells played an import.ant role in the prevention of the development of T1D in α-GalCer-treated NOD mice.
作者 李卫鹏 袁超 陶新全 王福庆
机构地区 蚌埠医学院第一附院核医学科 上海交通大学医学院上海市免疫学研究所
出处 《放射免疫学杂志》 CAS 2009年第5期513-516,共4页 Journal of Radioimmanology
关键词 CD4+CD25+调节性T细胞 平均荧光强度 转录因子FOXP3 环磷酰胺 Α-GALCER CD4＋CD25＋T regulatory cells mean fluorescent intensity transcription fator FoxP3 CY α-GalCer
分类号 R392 [医药卫生—免疫学] R331.14 [医药卫生—人体生理学]
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参考文献14

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二级参考文献10

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放射免疫学杂志
2009年 第5期

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