摘要
赤霉酸(纯GA_3)20μg·ml^(-1)处理刺槐(Robinia pseudoacacia L.)实生苗48h后核糖核酸酶(RNase)的活力增加两倍左右,对实生苗的子叶、幼茎和幼根中RNase活力的刺激效应基本相同。5-氟尿嘧啶、亚胺环己酮及高浓度嘌呤霉素可强烈抑制GA_3刺激RNase活力的增加,而放线菌素D则显示出刺激作用。标记RNase的部分提纯,揭露了RNase活力的增加是由于它新合成的缘故。
There was about two-fold stimulation of RNase activity by the application of GA3 at 20ppm to Black Locust seedlings for 48 hours. Stimulative effect of GA3 on RNase activity to the cotyledons, young stems and young roots of seedlings was basically the same. The increase in activity was inhibited by the administration of 5-fluorouracil,cycloheximide and puromycin but stimulated by the administration of actinomycin D. It seems likely that actinomycin D treatments resulted in maintenance of high levels of enzyme activity by inhibiting the production of substances associated with enzyme decay. Purification of labelled RNase revealed that GA3 enhanced the RNase activity mainly by its fresh synthesis.
出处
《林业科学研究》
CSCD
北大核心
1990年第1期34-40,共7页
Forest Research
关键词
刺槐
实生苗
核糖核酸酶
赤霉酸
Black locust
gibberellic acid
ribonuclease
3H-leucine
