摘要
目的探讨肿瘤坏死因子(TNF)-α-308G/A、白细胞介素(IL)-6-174G/C基因多态性与呼吸道合胞病毒((RSV)毛细支气管炎易感性的关系。方法应用聚合酶链反应-限制性酶切片段长度多态性技术(PCR-RFLP)检测150例RSV毛细支气管炎患儿(病例组)和120例正常对照儿童(对照组)TNF-α-308G/A和IL-6-174G/C的基因多态性。结果①病例组TNF-α-308位点GG、GA、AA基因型频率分别为68.0%、28.0%、4.0%,G、A等位基因型频率分别为82.0%,18.0%;对照组TNF-α-308位点GG,GA,AA基因型频率分别为80.8%,19.2%,0,G、A等位基因型频率分别为90.4%,9.6%;两组基因型及等位基因频率差异有显著性意义,病例组A等位基因的频率明显高于对照组(χ2=5.665,7.726,均P<0.05);与G等位基因携带者相比,A等位基因携带者患RSV毛细支气管炎的风险增加了2.071倍(OR=2.071,P<0.05);②病例组和对照组IL-6-174G/C位点均只见GG基因型。结论TNF-α-308A等位基因与RSV毛细支气管炎的易感性相关,其可能是影响RSV毛细支气管炎发病的一个重要候选基因;温州地区汉族儿童未发现IL-6-174G/C基因多态性。
Objective To study the association of tumor necrosis factor （TNF）-α-308G/A and interleukin （IL）-6 -174G/C gene polymorphisms with the susceptibility of respiratory syncytial virus （RSV） bronchiolitis.Methods Polymerase chain reaction-restriction fragment length polymorphism （ PCR-RFLP） was used to identify the polymorphisms at position 308 of the TNF-α promoter and -174 of the IL-6 promoter in 150 children with RSV bronchiolitis and 120 healthy children （control group）.Results The frequency of GG,GA and AA genotypes of TNF-α-308 was 68.0%,28.0% and 4.0% respectively in the RSV group,while that was 80.8%,19.2% and 0 respectively in the control group. There were significant differences in the genotype frequencies between the two groups （χ2=5.665,P〈0.05）. The frequency of G and A alleles in the RSV group was 82.0% and 18.0% respectively,while that was 90.4% and 9.6% respectively in the control group. The frequency of A allele in the RSV group was significantly higher than that in the control group （χ2=7.726,P〈0.05）. Compared to the children carrying G allele,the children with A allele demonstrated a 2.071-fold increased risk of RSV bronchiolitis （OR=2.071,P〈0.05）. Only GG genotype was detected at position -174 of IL-6 in both groups.Conclusions TNF-α-308A is associated with the susceptibility of RSV bronchiolitis and it might be an important candidate gene for the development of RSV bronchiolits. IL-6-174G/C gene polymorphism is not found in children in Wenzhou area.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2009年第10期821-824,共4页
Chinese Journal of Contemporary Pediatrics
基金
浙江省教育厅科研基金(20041071)
